Antibacterial activity of Clidemia hirta leaf extract against pathogenic bacteria with variation of solvents
Abstract
Infectious disease is one of the health problems in society that is difficult to solve completely. One such infectious disease is diarrhea. Diarrhea is a disease that has
high mortality and morbidity. Diarrhea can be caused by various types of pathogenic
bacteria. One of the antibacterial potentials that can reduce the activity of pathogenic
bacteria is the leaf extract of Clidemia hirta. The purpose of this study was to determine the antibacterial potential of C. hirta leaves against pathogenic bacteria with a variety of solvents. The research method used is descriptive qualitative. The solvents used were ethanol, water and n-hexane with the agar diffusion method. The extraction of the active compound was carried out using the maceration method. Measurement of antibacterial activity by looking at the minimum inhibitory concentration of C. hirta leaf extract in inhibiting the tested bacteria. The test bacteria used were Vibrio cholerae ATCC 39315 and Bacillus cereus ATCC 14574. The results showed that the minimum inhibitory concentration value for ethanol and Aqueous extract was 15,625 mg / ml for both types of bacteria, while for n-hexane solvent it was 62.5 mg/mL for V. cholerae ATCC 39315 and 250 mg/mL for B. cereus ATCC 14574. The conclusion is that ethanol extract is included in the category of very strong antibacterial activity, while Aqueous and n-hexane are categorized as strong in inhibiting the growth of pathogenic bacteria.
Full Text:
PDFReferences
Abdellaoui, S.E.l., Destandau, E., KrolikiewiczRenimel, I., Cancellieri, P., Toribio, A., Jeronimo-Monteiro, V., Landemarre, L., André, P., Elfakir, C. (2014) Centrifugal partition chromatography for antibacterial bio-guided fractionation of Clidemia hirta roots. Separat. Purif. Technol., 123: 221–228
Breiding, M.J. (2014) 肌肉作为内分泌和旁分泌器官 HHS Public Access. Physiol. Behav., 63(8): 1–18
Carroll, L.M., Wiedmann, M., Mukherjee, M., Nicholas, D.C., Mingle, L.A., Dumas, N.B., Cole, J.A., Kovac, J. (2019) Characterization of
emetic and diarrheal Bacillus cereus strains from a 2016 foodborne outbreak using wholegenome sequencing: Addressing the microbiological, epidemiological, and bioinformatic challenges. Frontiers Microbiol.,10
Dirar, A.I., Alsaadi, D.H.M., Wada, M., Mohamed, M.A., Watanabe, T., Devkota, H.P. (2019) Effects of extraction solvents on total phenolic
and flavonoid contents and biological activities of extracts from Sudanese medicinal plants. South Afr. J. Botan., 120: 261–267
Dong, S., Yang, X., Zhao, L., Zhang, F., Hou, Z., Xue, P. (2020) Antibacterial activity and mechanism of action saponins from Chenopodium quinoa Willd. husks against foodborne pathogenic bacteria. Indust. Crops Prod., 149: 112350
Farhadi, F., Khameneh, B., Iranshahi, M., Iranshahy, M. (2019) Antibacterial activity of flavonoids and their structure–activity relationship: An update review. Phytother. Res., 33(1): 13–40
Jia, Z., Chen, A., Bao, F., He, M., Gao, S., Xu, J., Zhang, X., Niu, P., Wang, C. (2018) Effect of nisin on microbiome-brain-gut axis
neurochemicals by Escherichia coli-induced diarrhea in mice. Micr. Pathog., 119: 65–71
Kurokawa, S., Kishimoto, T., Mizuno, S., Masaoka, T., Naganuma, M., Liang, K. ching, Kitazawa, M., Nakashima, M., Shindo, C., Suda, W.,
Hattori, M., Kanai, T., Mimura, M. (2018) The effect of fecal microbiota transplantation on psychiatric symptoms among patients with irritable bowel syndrome, functional diarrhea and functional constipation: An open-label observational study. J. Affect. Disord., 235: 506–512
Lopez, T., Corbin, C., Falguieres, A., Doussot, J., Montguillon, J., Hagège, D., Hano, C., Lainé, É. (2016) Influence de la composition du
milieu de culture sur la production de métabolites secondaires et les activités antioxydantes et antibactériennes des extraits produits í partir de cultures in vitro de Clidemia hirta L. Compt. Rend. Chimie, 19(9):1071–1076
Namdar, D., Voet, H., Ajjampura, V., Nadarajan, S., Mayzlish-Gati, E., Mazuz, M., Shalev, N., Koltai, H. (2019) Terpenoids and phytocannabinoids co-produced in Cannabis sativa strains show specific interaction for ell cytotoxic activity. Molecules, 24(17)
Nasution, M.Y., Restuati, M., Pulungan, A.S.S., Pratiwi, N., Diningrat, D.S. (2018) Antimicrobial activities of Centella asiatica leaf and root extracts on selected pathogenic micro-organisms. J. Med. Sci., 18(4): 198–204
Nasution, M.Y., Restuati, M., Syahputra, R.A., Pulungan, A.S.S. (2019) Antibacterial activity of Mandailing traditional plant leaves ethanol
extract of Mikania micrantha. J. Biosci. Res., 16(01): 793–797
Otsuka, Y., Agestika, L., Widyarani, W., Sintawardani, N., Yamauchi, T. (2019) Risk factors for undernutrition and diarrhea prevalence in an urban slum in Indonesia: Focus on water, sanitation, and hygiene. American J. Trop. Med. Hyg., 100(3), 727–732
Pulungan, A.S.S., Wuyung, W., Brata, W., Gultom, R. (2018) Antibacterial activity of the Taro ethanol extract against pathogenic bacteria. Sci. Stu. Res. Biol., 31–34
Breaden, R.C., Brooks, S.J., Murphy, H.T. (2012) The biology of Australia weeds. Plant Protec. Quart., 27(1): 2012
Yao, D.W., Yu, Z.Z., Li, N., Hou, Y.N., Xu, J.R., Yang, D.J. (2017) Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium. J. Zhejiang Univ.: Sci. B, 18(6): 474–480
DOI: https://doi.org/10.24114/jmns.v1i1.28465
Article Metrics
Abstract view : 241 timesPDF - 161 times
Refbacks
- There are currently no refbacks.
Editorial Office:
Faculty of Mathematics and Natural Sciences-Universitas Negeri Medan
Jl. Willem Iskandar / Pasar V, Medan, Sumatera Utara – Indonesia
Kotak Pos 1589, Kode Pos 20221
Telp. +6285260614736
E-mail : jmns@unimed.ac.id
Journal of Mathematics and Natural Sciences is licensed under a Creative Commons Attribute 4.0 Internasional Licence.