Antibacterial activity of Clidemia hirta leaf extract against pathogenic bacteria with variation of solvents

Evi Rahayu, Ahmad Shafwan S Pulungan

Abstract


Infectious disease is one of the health problems in society that is difficult to solve completely. One such infectious disease is diarrhea. Diarrhea is a disease that has
high mortality and morbidity. Diarrhea can be caused by various types of pathogenic
bacteria. One of the antibacterial potentials that can reduce the activity of pathogenic
bacteria is the leaf extract of Clidemia hirta. The purpose of this study was to determine the antibacterial potential of C. hirta leaves against pathogenic bacteria with a variety of solvents. The research method used is descriptive qualitative. The solvents used were ethanol, water and n-hexane with the agar diffusion method. The extraction of the active compound was carried out using the maceration method. Measurement of antibacterial activity by looking at the minimum inhibitory concentration of C. hirta leaf extract in inhibiting the tested bacteria. The test bacteria used were Vibrio cholerae ATCC 39315 and Bacillus cereus ATCC 14574. The results showed that the minimum inhibitory concentration value for ethanol and Aqueous extract was 15,625 mg / ml for both types of bacteria, while for n-hexane solvent it was 62.5 mg/mL for V. cholerae ATCC 39315 and 250 mg/mL for B. cereus ATCC 14574. The conclusion is that ethanol extract is included in the category of very strong antibacterial activity, while Aqueous and n-hexane are categorized as strong in inhibiting the growth of pathogenic bacteria.


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DOI: https://doi.org/10.24114/jmns.v1i1.28465

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