Exploration of extraceluller enzymes-producing bacteria symbiont Sponge Phorbas sp. from Nngge Island, Sibolga

Abstract

This study aims to determine the bacteria that is in symbiont sponges and has the potential to produce extracellular enzymes such as protease, amylase, cellulase and lipase. The sea sponge obtained from Ngge Island, Sibolga is a type of Phorbas sp. The enzymatic activity test was carried out. Bacterial isolates obtained from the sponge Phorbas sp. there are 7 bacterial isolates. Seven bacterial isolates had the ability to produce extracellular enzymes and proteolytic, amylolytic, lipolytic and cellulolytic bacteria. Proteolytic activity using test media enriched with skim milk (1%), amylolytic activity using starch-enriched test media (1%), cellulolytic activity using CMC enriched test media (1%) and lipolytic activity using Tween 80 enriched test media (1%). Based on the results of the research, seven isolates of sponge symbiont bacteria have enzymatic activity of amylase and cellulase, namely bacterial isolates P1, P2, P3, P4, P5, P6, P7 and six (6) bacterial isolates which have protease and lipase enzymatic activity are bacterial isolates P1, P2, P3, P4, P6, P7.