Isolation of Chitin from the Shell of the Blue Crab (Portunus pelagicus) and a Review of Its Potential as a Cholesterol - Lowering Agent
DOI:
https://doi.org/10.24114/ijcst.v9i2.71959Keywords:
chitin, cholesterol, Portunus pelagicusAbstract
The swimming crab (Portunus pelagicus) is a fishery commodity that produces large amounts of shell waste and has the potential to be used as a source of chitin. This study aims to isolate chitin from swimming crab shells through demineralization and deproteinization processes and then characterize it using FTIR, accompanied by a literature review regarding the potential of chitin as a cholesterol-lowering agent. The demineralization stage produced a yield of 35.07%, while the yield of chitin after deproteination reached 87.10%. FTIR analysis showed typical chitin absorption bands, namely N–H stretching (3255 cm⁻¹), Amide I (1621 cm⁻¹), Amide II (1552 cm⁻¹), C–O–C asymmetric stretching (1154 cm⁻¹), and β-(1→4) glycosidic bonds (872.8 cm⁻¹), which confirmed the formation of the chitin structure. Based on a literature review, chitin and chitosan have the potential to lower cholesterol levels through bile acid binding and reduced lipid absorption. Therefore, chitin from crab shell waste is suitable for development as a raw material for bioactive chitosan, although further biological testing is needed
References
1. Purhadi, P., Purnanto, N. T. P., & Natassia, K. (2020). Pengaruh konsumsi makanan yang mengandung lemak jenuh terhadap peningkatan kadar kolesterol di Desa Ngabenrejo. Jurnal TSNers, 5(1), 60–66.
2. Yogeswara, P. A., Setyowati, E. R., Ruqayyah, S., & Wiatma, D. S. (2023). Pengaruh indeks massa tubuh (IMT) dan kadar kolesterol dengan hipertensi di Puskesmas Gerung Kabupaten Lombok Barat Nusa Tenggara Barat. Jurnal Ners, 7, 744–752).
3. Kementerian Kesehatan Republik Indonesia. (2018). Laporan nasional Riskesdas 2018. Kemenkes RI.
4. Affandhy, A. K., Sudarjat, H., Hilmi, I. L., Shadrina, J. A., & Dzannuba, F. L. D. L. (2025). Literature review: Efektivitas dan efek samping PCSK9 inhibitor dibandingkan dengan golongan statin pada pasien penderita kolesterol. Jurnal Sehat Mandiri, 20, 66–79.
5. Maharani, P. F., & Susanti, R. (2022). Aplikasi kitosan cangkang rajungan (Portunus pelagicus) dalam ransum terhadap profil lipid darah itik. Life Sciences, 11, 184–191.
6. Irma, W., Karyani, M. S., & Amarlita, D. M. (2023). Analisis kitin dari cangkang kepiting rajungan (Portunus pelagicus). Horizons: Indonesian Journal of Multidisciplinary, 1, 105–116.
7. Andriani, Y., Lazuardi, T. A., Cahya, A. D., & Aisyah. (2025). Review literature: Pengolahan bahan pakan asal Crustacea sebagai kitin dan kitosan. Journal of Fish Nutrition, 5, 1–15.
8. Xu, W., Mohan, A., Pitts, N. L., Udenigwe, C., & Mason, B. (2020). Bile acid-binding capacity of lobster shell-derived chitin, chitosan and chitooligosaccharides. Food Bioscience, 33, 100476.
9. Hadi, K. S. (2020). Kemampuan kitin dari cangkang kepiting bakau (Scylla spp.) dalam menurunkan kadar kolesterol jeroan sapi. Program Studi Pendidikan Biologi, FKIP, Universitas Syiah Kuala, 5, 324–329.
10. Nadia, L. M. H., Huli, L. O., & Nadia, L. A. R. (2018). Pembuatan dan karakterisasi kitosan dari cangkang rajungan (Portunus pelagicus) asal Sulawesi Tenggara. Journal of Fish Protech, 1, 77–84.
11. Mahatmanti, F. W., Kusumastuti, E., Jumaeri, J., Susiyanti, A., Handayani, U. H., & Dirgantari, P. S. (2022). Memanfaatkan limbah menjadi material bernilai tambah. Buku Chapter Kimia, 32, 1–38.
12. Fernando, L. A. T., Poblete, M. R. S., Ongkiko, A. G. M., & Diaz, L. J. L. (2016). Chitin extraction and synthesis of chitin-based polymer films from Philippine blue swimming crab (Portunus pelagicus) shells. Procedia Chemistry, 19, 462–468.
13. Imtihani, H. N., Agung, R., & Nisa, S. (2020). Biopolimer kitosan dan penggunaannya.
14. Dompeipen, E. J., Kaimudin, M., & Dewa, R. P. (2016). Isolasi kitin dan kitosan dari limbah kulit udang. Balai Riset dan Standardisasi Industri Ambon, 12, 32–38.
15. Mahardika, R. G., Jumnahdi, M., & Widyaningrum, Y. (2020). Deasetilasi kitin cangkang rajungan (Portunus pelagicus) menjadi kitosan menggunakan iradiasi microwave. 3, 149–158.
16. Intan, S., Fadila, N., Athirah, F. A., Perdani, M. S., & Pambudi, T. (2024). Preliminary study of chitin extraction from crickets (Acheta domesticus) using deep eutectic solvent. 6, 268–282.
17. Imonek, J., & Ová, H. B. (2005). Effect of dietary chitin and chitosan on cholesterolemia of rats. 491, 491–499.
18. Azuma, K. et al. Effects of surface-deacetylated chitin nanofibers in an experimental model of hypercholesterolemia. Int. J. Mol. Sci. 16, 17445–17455 (2015).
