ANTIBACTERIAL ACTIVITIES OF RUKAM LEAVES (Flacourtia rukam Zoll. & Moritzi) AGAINST Staphylococcus aureus AND Escherichia coli

Henri Henri, Rahmad Lingga

Abstract


Traditional herbal medicines are now attracted significant attention used as the basis for modern medicines, including the plant of rukam (Flacourtia rukam) from the Flacourtiaceae family which is known by the public as medicine. This research aimed to explore the potential antibacterial activity for the plant of rukam (F.rukam) against bacterial Staphylococcus aureus and Escherichia coli. This research method was to first test the secondary metabolite content of F. rukam by using four solvents. The antibacterial activity extract from leaves of F. rukam was examined against S. aureus and E. coli. The antibacterial activity was assessed in the concentration include 20%, 40%, 60%, 80%, and 100% by disc diffusion method. Phytochemical test results such as phenolics, flavonoids, saponins, steroids, and alkaloids are secondary metabolites of the F. rukam plant. The highest zone of inhibition is at a concentration of 60% using methanol solvent, which is an average of 8.95 ± 1.84 in S. aures isolates, while the E. coli bacterial isolates have an average of 9.03 ± 0.95 . This result was different from using ethanol solvent where the highest inhibition zone was at a concentration of 20%, respectively 7.73 ± 2.79 in S. aureus isolates and 6.61 ± 2.18 in E. coli bacteria isolates. Antibacterial efficacy shown by this plant F. rukam provides a scientific basis and thus validates traditional use.


Keywords


Antibacterial activity, Escherichia coli, Flacourtia rukam, Staphylococcus aureus

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Al-Saleem, M. S., Awaad, A. S., Alothman, M. R., & Alqasoumi, S. I. 2018. Phytochemical standardization and biological activities of certain desert plants growing in Saudi Arabia. Saudi Pharmaceutical Journal, 26(2), 198–204.

Appapalam, S. T., & Panchamoorthy, R. 2017. Aerva lanata mediated phytofabrication of silver nanoparticles and evaluation of their antibacterial activity against wound associated bacteria. Journal of the Taiwan Institute of Chemical Engineers, 78, 539–551.

Arasu, M. V., Arokiyaraj, S., Viayaraghavan, P., Kumar, T. S. J., Duraipandiyan, V., Al-Dhabi, N. A., & Kaviyarasu, K. 2019. One step green synthesis of larvicidal, and azo dye degrading antibacterial nanoparticles by response surface methodology. Journal of Photochemistry and Photobiology B: Biology, 190, 154–162.

Bhatia, P., Sharma, A., George, A. J., Anvitha, D., Kumar, P., Dwivedi, V. P., & Chandra, N. S. 2021. Antibacterial activity of medicinal plants against ESKAPE: An update. Heliyon, 7(2), e06310.

Biswas, B., Rogers, K., McLaughlin, F., Daniels, D., & Yadav, A. 2013. Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two gram-negative and gram-positive bacteria. International Journal of Microbiology, 1–7.

Bonjar, G. H. S., Aghighi, S., & Nik, A. K. 2004. Antibacterial and Antifungal Survey in Plants used in Indigenous Herbal-Medicine of South East Regions of Iran. Journal of Biological Sciences, 4(3), 405–412.

Candella, F. 2020. Analisis Total Fenolik, Flavonoid dan Aktivitas Antioksidan serta Antibakteri dari Ekstrak Kulit Batang Rukam (Flacourtia rukam). [skripsi]. Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam: Universitas Sriwijaya.

Christin, M. 2015. Buah rukam atau rukem. https://www.biodiversitywarriors.org.

De Zoysa, M. H. N., Rathnayake, H., Hewawasam, R. P., & Wijayaratne, W. M. 2019. Determination of in Vitro Antimicrobial Activity of Five Sri Lankan Medicinal Plants against Selected Human Pathogenic Bacteria. International Journal of Microbiology, 1–8.

Deshmukh, B. S., Waghmode, A., & Arts, A. 2011. Role of wild edible fruits as a food resource: Traditional knowledge. International Journal of Pharmacy & Life Sciences, 2(7), 919–924.

Dike-Ndudim, J. ., Anyanwu, G. ., Egbuobi, R. ., Okorie, H. ., Udujih, H. ., Nwosu, D. ., & Okolie, N. J. . 2016. Anti-bacteria and phytochemical potential of Moringa oleifera leaf extracts on some wound and enteric pathogenic bacteria. European Journal of Botany, Plant Sciences and Phytology, 3(1), 50–60.

Fadiyah, I., Lestari, I., & Mahardika, R. G. 2020. Kapasitas Antioksidan Ekstrak Buah Rukam (Flacourtia rukam) Menggunakan Metode Microwave Assisted Extraction (MAE). Indo. J. Chem. Res., 7(2), 107–113.

Farasat, M., Khavari-Nejad, R. A., Nabavi, S. M. B., & Namjooyan, F. 2014. Antioxidant activity, total phenolics and flavonoid contents of some edible green seaweeds from northern coasts of the Persian gulf. Iranian Journal of Pharmaceutical Research, 13(1), 163–170.

Godstime, O., Felix, E., Augustina, J., & Christopher, E. 2014. Mechanisms of Antimicrobial Actions of Phytochemicals against Enteric Pathogens - A Review. Journal of Pharmaceutical, Chemical and Biological Sciences, 2(2), 77–85.

Haryati, N., Saleh, C., & Erwin. 2015. Uji Toksisitas dan Aktivitas Antibakteri Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium Myrtifolium Walp.) Terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli. Jurnal Kimia Mulawarman, 13(1), 35–40.

Hassan, H. U., Murad, W., Tariq, A., & Ahmad, A. 2014. Ethnoveterinary study of medicinal plants in Malakand Valley, District Dir (Lower), Khyber Pakhtunkhwa, Pakistan. Irish Veterinary Journal, 67(1), 1–6.

Kalaiselvi, M., Subbaiya, R., & Selvam, M. 2013. Synthesis and characterization of silver nanoparticles from leaf extract of Parthenium hysterophorus and its anti-bacterial and antioxidant activity. International Journal of Current Microbiology and Applied Sciences, 2(6), 220–227.

Karmilasanti, K., & Supartini, S. 2011. Keanekaragaman Jenis Tumbuhan Obat Dan Pemanfaatannya di Kawasan Tane’ Olen Desa Setulang Malinau, Kalimantan Timur. Jurnal Penelitian Dipterokarpa, 5(1), 23–38.

Lim, T. K. 2012. Flacoutia rukam. Edible Medicinal and Non-Medicinal Plants, 5, 776–779.

Mahalakshmi, N., Dhanasekaran, S., Ravi, C., & Lingathurai, S. 2016. In‐vitro antimicrobial activities of Pongamia glabra and Phyllanthus niruri. South Indian Journal Of Biological Sciences, 2(2), 236–244.

Mahanisa, A. S. 2019. Flavonoid apigenin dari ekstrak daun tumbuhan rukam (Flacourtia rukam) serta uji aktivitas antioksidan dan antibakteri. [skripsi]. Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam: Universitas Sriwijaya.

Mahmud, J., & Khan, R. A. 2018. Characterization of Natural Antimicrobials in Food System. Advances in Microbiology, 8(11), 894–916.

Mariana, L., Andayani, Y., & Gunawan, R. 2013. Analisis Senyawa Flavonoid Hasil Fraksinasi Ekstrak Diklorometana Daun Keluwih. Chemistry Progress, 6(2), 50–55.

Marliana, S. D., Suryanti, V., & Suyono. 2005. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq . Swartz .) dalam Ekstrak Etanol. Biofarmasi, 3(1), 26–31.

Marnoto, T., Haryono, G., Gustinah, D., & Putra, F. A. 2012. Ekstraksi Tannin Sebagai Bahan Pewarna Alami Dari Tanaman Putrimalu (Mimosa Pudica) Menggunakan Pelarut Organik. Reaktor, 14(1), 39–45.

Martini, N. L., Dwiyani, R., & Pradnyawathi, N. L. M. 2015. Identifikasi dan Karakterisasi Sumber Daya Genetik Buah-buahan di Kabupaten Bangli. Agrotrop: Journal on Agriculture Science, 5(2), 179–186.

Mostafa, A. A., Al-Askar, A. A., Almaary, K. S., Dawoud, T. M., Sholkamy, E. N., & Bakri, M. M. 2018. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences, 25(2), 361–366.

Neelamkavil, S. V., & Thoppil, J. E. 2016. Evaluation of the Anticancer Potential of the Traditional Medicinal Herb. South Indian Journal of Biological Sciences, 2(1), 41–45.

Ng, C. Y., Yen, H., Hsiao, H. Y., & Su, S. C. 2018. Phytochemicals in skin cancer prevention and treatment: An updated review. International Journal of Molecular Sciences, 19(4), 1–24.

Parija, S. C. (2012). Textbook of Microbiology & Immunology 2 nd Edition. Pudhucerry, India: Elsevier.

Pendleton, J. N., Gorman, S. P., & Gilmore, B. F. 2013. Clinical relevance of the ESKAPE pathogens. Expert Review of Anti-Infective Therapy, 11(3), 297–308.

Perry, C. C., Weatherly, M., Beale, T., & Randriamahefa, A. 2009. Atomic forcemicroscopy study of the antimicrobial activity of aqueous garlic versus ampicillin against Escherichia coli and Staphylococcus aureus. Journal of the Science of Food and Agriculture, 89(6), 958–964.

Putri, D. V., Lestari, F., & Widiya, M. 2019. Uji Daya Antibakteri Sari Pati Daun Rukam (Flacourtia rukam) terhadap Zona Hambat Escherichia coli. Jurnal Biosilampari : Jurnal Biologi, 2(1), 23–28.

Rasingam, L. 2012. Ethnobotanical studies on the wild edible plants of Irula tribes of Pillur Valley, Coimbatore district, Tamil Nadu, India. Asian Pacific Journal of Tropical Biomedicine, 2(3), 1493–1497.

Setiabudi, D. ., & Tukiran. 2017. Uji Skrining Fitokimia Ekstrak Metanol Kulit Batang Tumbuhan Klampok Watu (Syzygium litorale). UNESA Journal of Chemistry, 6(3), 155–160.

Shaikh, S., Fatima, J., Shakil, S., Rizvi, S. M. D., & Kamal, M. A. 2015. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences, 22(1), 90-101..

Sofowora, A. 1993. Screening plants for bioactive agents. In medicinal plants and traditional in Africa (2nd ed.). Ibadan, Nigeria: pectrum Books Ltd., Sunshine House.

Spellberg, B., Blaser, M., Guidos, R. J., Boucher, H. W., Bradley, J. S., Eisenstein, B. I., & Gilbert, D. N. 2011. Combating antimicrobial resistance: Policy recommendations to save lives. Clinical Infectious Diseases, 52(5), 397–428.

Susanto, Sudrajat, D., & Ruga, R. 2012. Studi kandungan bahan aktif tumbuhan meranti merah (Shorea leprosula Miq) sebagai sumber senyawa antibakteri. Mulawarmnan Scientific, 11(2), 181–190.

Tan, S. L., Lee, H. Y., & Mahyudin, N. A. 2014. Antimicrobial resistance of Escherichia coli and Staphylococcus aureus isolated from food handler’s hands. Food Control, 44, 203–207.

Thakur, M., Melzig, M. F., Fuchs, H., & Weng, A. 2011. Chemistry and pharmacology of saponins: special focus on cytotoxic properties. Botanics: Targets and Therapy, 19–29.

Trease, G. ., & Evans, W. 2002. Farmakognosi (15th ed.). Berlin: Springer.




DOI: https://doi.org/10.24114/jbio.v7i3.21595

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JBIO : Jurnal Biosains (The Journal of Biosciences), Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan. ISSN 2443-1230 (print) dan ISSN 2460-6804 (online)

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