PENGARUH EKSTRAK ETANOL Plectranthus amboinicus Lour Spreng TERHADAP BERAT BADAN DAN BERAT RELATIF ORGAN TIKUS YANG DINDUKSI KANKER KULIT DENGAN DMBA

Melva Silitonga, Erlintan Sinaga, Pasar Maulim Silitonga

Abstract


Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun Plectranthus amboinicus terhadap  berat dan berat relatif organ dan berat badan tikus yang diinduksi kanker kulit dengan DMBA. Digunakan 24 ekor tikus dan dibagi menjadi empat kelompok yaitu K-, K+, T1 dan T2. K- yaitu kelompok kontrol diberi CMC 0.5%. K+  diberi DMBA 175 µg, T1 dan T2 diberi DMBA 175 µg dan EEP sebanyak 250 dan 500 mg/kg berat badan. Induksi DMBA diterapkan secara topical dibagian sisi posterior punggung dengan luas 3×3cm. DMBA dilarutkan dalam 0.1 ml aceton dioleskan. seminggu dua kali selama delapan minggu. Pada minggu ke sembilan, diberikan EEP setiap hari selama empat minggu pada kelompok T1 dan T2. Selama penelitian semua tikus diberi pakan dan minum secara ad libitum. Pada minggu ke 13 tikus dibunuh lalu dibedah untuk memperoleh organ ginjal, paru, hati dan limpa. Data yang diperoleh dianalisis dengan Anova. Hasil penelitian menunjukkan induksi kanker kulit dengan DMBA meningkatkan berat jantung, paru dan ginjal. Pemberian EEP menurunkan berat organ tersebut  menjadi sama dengan kontrol. DMBA meningkatkan berat relatif organ jantung, ginjal, paru-paru dan hati. EEP menurunkan berat relatif organ.  Pemberian DMBA menurunkan berat  badan mingguan pada tikus perlakuak K+, T1 dan T2. Pemberian EEP meningkatkan berat badan tikus yang diinduksi kanker kulit dengan DMBA


Keywords


Plectranthus amboinicus, berat relative organ, berat badan, DMBA

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DOI: https://doi.org/10.24114/jbio.v7i3.23288

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JBIO : Jurnal Biosains (The Journal of Biosciences), Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan. ISSN 2443-1230 (print) dan ISSN 2460-6804 (online)

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