ANTIMICROBIAL ACTIVITY TEST OF MEDICINAL PLANT EXTRACT USING ANTIMICROBIAL DISC AND FILTER PAPER AGAINST Staphylococcus aureus : CLEAR ZONE RESULTS COMPARISON

Muhammad Taufiq Hidayat, Maharani Pertiwi Koentjoro, Maharani Pertiwi Koentjoro, Endry Nugroho Prasetyo, Endry Nugroho Prasetyo

Abstract


Ekstrak tumbuhan mengandung metabolit sekunder berupa flavonoid dan senyawa fenolik yang memiliki aktivitas antibiotik. Uji aktivitas antibiotik dapat dilakukan dengan metode cakram Kirby-Bauer yang aktivitas antibiotiknya ditunjukkan dengan terbentuknya zona bening. Penelitian ini dilakukan dengan menggunakan ekstrak  daun Moringa oleifera  dan  kladode Opuntia cochenillifera  yang diekstrak menggunakan etil asetat sebagai pelarut. Uji aktivitas antibiotik terhadap  bakteri Staphylococcus aureus  dilakukan pada media Mannitol Salt Agar dan Luria Bertani Agar. Cakram Kirby-Bauer yang digunakan adalah Antimicrobial Disc Oxoid® dan Filter Paper. Hasil penelitian ini menunjukkan bahwa penggunaan Antimicrobial disc Oxoid® dan Filter Paper tidak menunjukkan perbedaan secara statistik pada hasil zona bening (t-value = 0,45; p-value = 0,655; dengan α = 95%). Sementara itu, hasil Clear Zone pada Mannitol Salt Agar memberikan hasil yang lebih baik daripada Luria Bertani Agar dan signifikan secara statistik (nilai-t = 2,46; nilai-p = 0,02; dengan α = 95%). Temuan ini menunjukkan bahwa kertas saring dapat menjadi alternatif yang murah untuk uji antibiotik dengan hasil yang tidak berbeda secara signifikan dibandingkan dengan cakram antibiotik komersial. Namun, uji antibiotik terhadap Staphylococcus aureus lebih baik dilakukan pada Mannitol Salt Agar dibandingkan dengan Luria Bertani Agar.


Keywords


Antibiotic Test, Antimicrobial Disc, Kirby-Bauer Disc Method, Mannitol Salt Agar, Staphylococcus aureus

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References


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DOI: https://doi.org/10.24114/jbio.v10i2.43276

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JBIO : Jurnal Biosains (The Journal of Biosciences), Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan. ISSN 2443-1230 (print) dan ISSN 2460-6804 (online)

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