PENGEMBANGAN METODE ANALISIS SPEKTROFOTOMETRY UNTUK PENENTUAN KOLESTEROL DI DALAM MAKANAN TRADISIONAL

Manihar Situmorang, P Maulim Silitonga, Isnaini Nurwahyuni, Linda Sari Siregar

Abstract


The development of analytical UV-Vis spectrophotometric method for
the determination of cholesterol is explained. The assay performed by enzymatic
catalytic reaction ·using cholesterol oxidase (COx) to . oxidise cholesterol into
cholestenon and hydrogen peroxide followed by catalytic reduction of hydrogen
peroxide with o-dianicidine by using enzyme peroxidase (POx) to produce red
colour reduced o-dianicideine that can be measured at ii. 520 nm. The modified
method gave sensitive response to cholesterol, where the detection linearity to
cholesterol lies between 0.01-5.0 mM cholesterol, slope 0.158 aulmM cholesterol,
and the detection limit of 0~01 mM cholesterol. The method has been applied for the
determination of cholesterol in traditional food samples, where the concentrations of
cholesterol in food are lies between 0.052-0.799 mM cholestero)


Keywords


Analisis, kolesterol, makanan tradisionil, spektrofotometry, reaksi enzimasi

Full Text:

PDF

References


Amundson, D.M. dan Zhou, M.J., (1999), Fluorometric method for the enzymaticdetermination of cholesterol, Journal Biochemical Biophysical Method 38: 43-52.

Assmann, G., Cullen, P., Jossa, F., Lewis, dan Mancini, M., (1999), Coronary heartdisease: Reducing the risk - The scientificbackground to primary and secondaryprevention of coronary heart disease worldwide view, Arteriosclerosis Trombosisand Vascular Biology 19: 1819-1824.

Assmann, G., Cullen, P., [ossa, F., Lewis, dan Mancini, M., (1999), Coronary heartdisease: Reducing the risk - The scientificbackground to primary and secondaryprevention of coronary heart disease worldwide view, Arteriosclerosis Trombosisand Vascular Biology 19: 1819-1824

Botsoglou, N., Fletouris, D., Psomas, I., dan

Mantis, A., (1998), Rapid gas chromatographic method for simultaneou sdetermination of cholesterol and alpha- tocopherol in eggs, Journal of AOAC International 81: 1177-1183:

Martin, S.P., Lamb, D.J., Lynch, J.M. dan Reddy, S.M., (2003), Enzyme-based determination of cholesterol using thequartz crystal acoustic wave sensor,

Analytica Chimica Acta 487(1): 91-100. Park, Y.W., (1999), Cholesterol contents of US and imported goat milk cheeses as quantified by different colorimetric methods, Small Ruminant Research 32: 77-82

Singh, S., Chaubey, A. dan Malhotra, B.D., (2004), Arnperometric cholesterol biosensor based on immobilized cholesterol esterase

and cholesterol oxidase on conducting polypyrrole films, Analytica Chimica Acta 502(2): 229-234. Situmorang, M. dan Nurwahyuni, I., (2001), Immobilasi enzim dalam reaktor untuk

penentuan kolesterol serum, Majalah Kedokteran Nusaniara 34: 84-89

Situmorang, M., (2010), Pengembangan Biosensor Untuk Menguji KualitasMakanan Dan Minuman, Seminar Nasionaldan Rapat Tahunan (SEMIRATA 2010), di

Pekan Barn

Situmorang, M., Sinaga, B.J., Siturnorang, I.F.,

dan Marpaung, F.M.T., (2009), Rancang

Bangun Strip Biosensor Untuk Penentuan

Jumal Saintika I Volume 12 I Nomor II I September 2012 Asam Urat Dalam Daging Dan Ikan

Kaleng, [urnal Sain Indonesia 33(1): 1-7.

Situmorang, M., Siregar, T.H., Simatupang, dan Krisnawati, H., (2008),

Spektrofotometri Penentuan Asam Urat

Dalam Daging Dan Makanan Kaleng Menggunakan Pengabsorbsi Dianisidin, ]urnal Sain Indonesia 32(2): 109-115

Situmorang, M., Alexander, P.W. dan Hibbert,

D.B., (1998), Flow Injection Potentiometry

for Enzymatic Assay of Cholesterol With Tungsten Electrode Sensor, Talanta 49(3):

-649

Situmorang, M .. Silitonga, P.M .. Nurwahyuni,

I, (2011), Pengembangan Biosensor Sebagai

Instrumen Analisis Untuk Menguji Kualitas

Makanan Dan Minuman, Laporan Penelitian,

FMIPA Universitas Negeri Medan.

Situmorang, M .. Silitonga, P.M .. Nurwahyuni,

I., Butar-butar, A. dan Nainggolan, M.,

(2007), Rancang Bangun Biosensor

Elektrokimia Dalam Sistem Flow Injeksi

Analisis Untuk Penentuan Kolesterol di

Dalam Makanan dan Minuman, ]urnal Sain

Indonesia 30(4): 125-130. ·

Situmorang, M .. Simanjuntak, E.P., dan Silaen,

D, (2010), Pengembangan Metode Analisis

Spektrofotometry Melalui Reaksi Enzimasi

Untuk Penentuan Glukosa Di Dalam Buah-

Buahan, ]urnal Sain Indonesia 34(3): 8-14.

Sniderman, A.D. dan Cianflone, K., (1999),

Lipids and vascular. disease: what we do

and do not know, Clinica Chimica Acta 286:

-22.


Article Metrics

Abstract view : 326 times
PDF - 3183 times

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
slot gacor slot