PERTUMBUHAN OKULASI JERUK KEPROK BRASTEPU (Citrus nobilis Var. Brastepu) MENGGUNAKAN JERUK ASAM SEBAGAI BA TANG BA WAH

Isnaini Nurwahyuni, Justin A Napitupulu, Rosmayati ., Fauziah Harahap

Abstract


The growth of Citrus nobilis Var. Brastepu budding that is free from
Citrus Vein Phloem Degeneration (CVPD) is explained. The method is conducted
by taking of bud stick from a selected healthy citrus Brastepu plant and is then
grafted into a sour orange (Citrus auraniium) as mother trees via budding method.
The research is conduted in Desa Bukit, Brastagi North Sumatra, Indonesia.
Optimum conditions on the budding technique have been obtained to produce good
qualih; seedling of Citrus nobilis Var. Brastepu. Growth characteristics of the plant
in various treatment conditions have been studied based on the growth leaves, stem,
and branches. Analysis of the DNA using RAPD-PCR method has confirmed that
all new seedling produced in the propagation method are free from CVPD.


Keywords


okulasi, jeruk keprok, Citrus nobilis, CVPD, Isolasi DNA, PCR.

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