PERTUMBUHAN OKULASI JERUK KEPROK BRASTEPU (Citrus nobilis Var. Brastepu) MENGGUNAKAN JERUK ASAM SEBAGAI BA TANG BA WAH

Isnaini Nurwahyuni, Justin A Napitupulu, Rosmayati ., Fauziah Harahap

Abstract


The growth of Citrus nobilis Var. Brastepu budding that is free from
Citrus Vein Phloem Degeneration (CVPD) is explained. The method is conducted
by taking of bud stick from a selected healthy citrus Brastepu plant and is then
grafted into a sour orange (Citrus auraniium) as mother trees via budding method.
The research is conduted in Desa Bukit, Brastagi North Sumatra, Indonesia.
Optimum conditions on the budding technique have been obtained to produce good
qualih; seedling of Citrus nobilis Var. Brastepu. Growth characteristics of the plant
in various treatment conditions have been studied based on the growth leaves, stem,
and branches. Analysis of the DNA using RAPD-PCR method has confirmed that
all new seedling produced in the propagation method are free from CVPD.


Keywords


okulasi, jeruk keprok, Citrus nobilis, CVPD, Isolasi DNA, PCR.

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References


Almeida, R.P.P., Nascimento, F.E., Chau, J.,

Prado, S.S., Tsai, CW, Lopes, S.A.,

dan Lopes, J.R.S., (2008), Genetic

Structure and Biology of Xylella

fastidiosa Strains Causing Disease in

Citrus and Coffee in Brazil, Applied

And Environmental Microbiology, 74

(12), 3690-3701.

Bove, J.M., 2006. Huanglongbing: A

destructive, newly-emerging,

century old disease of citrus.

Journal of Plant Pathology 88(1): 7-

Do Carmo, T.D; Luc,

Cristina, M.E.;

Takao, Y.P.;

D.J.; Eveillard, S.;

de Jesus, J.WC.;

Aparecido, L.S.;

Beozzo, B.R.; Juliano, A.A.; Saillard,

C.; Bove, JM., (2005), Citrus

huanglongbing in Sao Paulo State,

Brazil: PCR detection of the

'Candidatus' Liberibacter species

associated with the disease, Mol

Cell Probes. 19(3): 173-179.

Kim, J.S., dan Wang, N., (2009)

Characteristization of copy numbers of 16 S DNA and 16 S RNA of

Candidatus Liberibacter asiaticus

and the implication in detection in

planta using quantitative PCR. BMC

Research Notes 2: 37-40.

Prastowo, N.R.; Roshetso, J.M.; Manurung,

G.E.S.; Nugraha, E.; Julian, J.M. dan

Harum, F., (2006), Teknik

pembibitan dan perbanyakan

vegetatif tanaman buah. World

Agroforestry centre (ICRAF). Bogor.

p.87.

Rieger, M., (2006), Introduction to Fruit

Crops. Hawthorn Food Products

Press, NY. p.462.

Sanford M.T. 1992. Pollination of citrus by

honey bees. UF/IFAS document

RF AA092. Available online.

Su, H.J. (2008), Production and cultivation

of virus free citrus saplings for

citrus rehabilitation in Taiwan.,

APCoAB. New Delhi. India.

Sumaatrnaja, G., (2001), Teknik

perbanyakan tanaman jeruk manis

di Kabupaten Bengkulu Selatan.

Laporan Teknis PUSLIT-Biologi-

LIPI. Jakarta.

Williamson, J.G., dan Jackson, L.K., (1994),

Citrus propagation. University of

Florida, Fact Sheet HS-86, June 1994.

http://ultimatecitrus.com


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