IDENTIFIKASI BAKTERI ASAM LAKTAT PADA JEROAN PEDA IKAN KEMBUNG (Rastrelliger sp.) SEBAGAI AGEN PROBIOTIK
Abstract
This study aims to determine the types and characteristics of lactic acid bacteria contained in the innards Peda Mackerel. The research was conducted in April to June 2013 in the Microbiology Laboratory Biology Departement FMIPA UNIMED. This study uses descriptive. Sampling techniques in aseptic. Materials used are lactic acid bacteria taken from the innards Peda Mackerel and grown on MRS medium (de Man Rogosa Sharpe). Based on the research results of the identification and characterization of lactic acid bacteria in Peda Mackerel using Test morphology, Gram staining and Biochemical tests of the obtained 2 types of lactic acid bacteria on the innards Peda Mackerel. The genus of lactic acid bacteria found include Lactobacillus and Streptococcus. Characteristics in general is a form of cocci and bacilli, Gram positive, catalase negative, non motile, the optimum temperature of 20 L 0C-400C and both have type homofermentatif fermentation. Genus agency that acts as a probiotic is genus Lactobacillus, while the genus Streptococcus can not act as an agency probiotics because it does not have the ability of to survive in the digestive tract.
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Desniar, Peornomo D, Wijatur
W.
(2009).
Pengaruh
Konsentrasi Garam pada Peda
Ikan Kembung (Rastrelliger sp.)
Dengan Fermentasi Spontan.
Departemen Teknologi Hasil
Perairan Fakultas Perikanan
dan Ilmu Kelautan Institut
Pertanian Bogor. Bogor. Hal:
Hutkins RW.
(2006).
Microbiology and Technology of
Fermented Foods. USA: IFT
Press. Blackwell Publishing.
Anonim, (2012). Hasil
Perikanan:
http://fpik.bunghatta.ac.idfiles
downloadsEbook
_Dasar_Dasar_%_20_Teknolog
i_%_20_Hasil_%_20_Perikanan_bab_6.pdf (Diakses Desember
.
Ensminger, M. E. (1990). Feeds
and Nutrition. The Ensminger
Publishing
Company.
California.
Salle, A.J. (1979). Fundamental
Principles of bacteriology. Tata
McGraw-Hill
Publishing
Company Ltd, New Delhi.
Buckle, K. A., R. A. Edwards, G.
H. Fleet and Wooton. (1987).
Ilmu Pangan. Universitas
Indonesia Press,
Jakarta
(diterjemahkan oleh H.
Purnomo dan Adiono).
Rahayu, E dan Nur, S. P., (2002),
Isolasi dan Seleksi Lactobacillus
yang berpotensi sebagai agensi
Probiotik, Agritech Vol.23 No.2
Hal 67-74.
Rahayu. E. dan Nur, S. P.,
(2004). Pediococcus acidilactici F//
penghasil bakteriosin sebagai
agensia biokontrol E. Coli dan S.
aureus pada Sayuran Segar
Simpan Dingin. Agritech 24
(3):113-124.
Purwandhani, S. N. dan
Rahayu, E. S. (2003). Isolasi dan
Seleksi Lactobacillus yang
Berpotensi sebagai Agensia
Probiotik. Agritech 23 (2):67 -74.
Hartati, S dan E. Harmayani.
(2006). Preparasi Sel Kering
Lactobacillus sp DAD l3 dan
Kestabilannya sebagai bubuk Probiotik.
J. Mikrobiotogi
Indonesia. Vol I I (1) : I -4.
Umniyati, S.,
(2007),
Pengembangan Probiotik Bakteri
Asam Laktat dan Enzim
Kolesterol Reduktase dari Limbah
Kotoran Ayam yang Potensi
Menurunkan Kadar Kolesterol
Daging Ayam Broiler, Laporan
Hasil Penelitian, FMIPA UNY.
Ristiati, N P., (2000), Pengantar
Mikrobiologi Umum, Dirjen
Dikti, Jakarta.
Tarigan, J. (1988). Pengantar
Mikrobiologi. Dikti Depdikbud,
Jakarta.
Savitri, S. (2006). Isolasi dan
Karakterisasi
Bakteri
Halotoleran pada Peda Ikan
Kembung (Rastrelliger Sp.).
Program studi teknologi hasil
perikanan Fakultas perikanan
dan ilmu kelautan Institut
Pertanian Bogor.
Holt G. J., Krieg R. N, Sneath A.
H. P., Staley T. J., Williams T.
A., (I994). Bergey’s Manual of
Determinative Bacteorology
Second Edition, Williams and
Wilkins Awaverly Company,
USA.
Anonim, (2010). Bakteri
Menguntungkan:
http://
biologigozonz.blogspot.com
/2010/
bakteri-
menguntungkan.html (Diakses
Januari 2013).
Aswin., (2010). Lactobacillus Reuteri
Membawa Banyak Manfaat Bagi
Saluran Cerna, http:// www.
medical
jurnal.com/edisi-tahun-
/lactobacillus-reuteri-membawabanyak-manfaat
bagi-saluran-
cerna.html (Diakses Maret 2013).
Soemarno, (2000). Isolasi dan
Identifikasi Bakteri Klinik
Akademi Analis Kesehatan Yogya
karta, Departemen Kesehatan
Republik
Indonesia,
Yogyakarta.
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