PEMURNIAN ASAM LINOLEAT TERKONJUGASI HASIL SINTESA DARI RISINOLEAT CASTOR OIL DENGAN KROMATOGRAFI KOLOM SILIKAGEL PERAK NITRAT
Abstract
Conjugated Linoleic acid (CLA) was sepereted by using
colomn chromatography with silicagel – AgNO3 as stationary
phase. Seperation based on the differences reversibility of
coordination bond between double bond and silver ion. The
indreasing number of double bond and trans isomers more
strongly the molecule is retained. Gradien elution was carried out
by various n-hexane (H) and Acetone (A) (v/a) e. g: 97.5/2.5; 95/5;
90/10; 80/20; 70/30 and 60/40 in 8 vial x 2.5 mL. Each of fraction
was analyzd by using GC,FTIR, UV and GC-MS and It’s compare
with authentic CLA standard and the best was ratio (v/v) of eluen
(H/A) 95/5. The composition of product ratio of CLA/linoleic was
0,85 : 1 or 77.6 % compared to the authentic standar 1.45/1.
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