PEMURNIAN ASAM LINOLEAT TERKONJUGASI HASIL SINTESA DARI RISINOLEAT CASTOR OIL DENGAN KROMATOGRAFI KOLOM SILIKAGEL PERAK NITRAT
Abstract
Conjugated Linoleic acid (CLA) was sepereted by using
colomn chromatography with silicagel – AgNO3 as stationary
phase. Seperation based on the differences reversibility of
coordination bond between double bond and silver ion. The
indreasing number of double bond and trans isomers more
strongly the molecule is retained. Gradien elution was carried out
by various n-hexane (H) and Acetone (A) (v/a) e. g: 97.5/2.5; 95/5;
90/10; 80/20; 70/30 and 60/40 in 8 vial x 2.5 mL. Each of fraction
was analyzd by using GC,FTIR, UV and GC-MS and It’s compare
with authentic CLA standard and the best was ratio (v/v) of eluen
(H/A) 95/5. The composition of product ratio of CLA/linoleic was
0,85 : 1 or 77.6 % compared to the authentic standar 1.45/1.
Keywords
Full Text:
PDFReferences
Adolf .R. O; A. Menzel and V. D. Taran;
; Analysis of Conjugated Linolec
Acid Enriched Triacylglycerol Mixture by Isocratic Silver – Ion High
Performance Liquid Chromatography;
J. Chrom. A 953 : 293 – 297.
Albers . R; R. P. J. Van der Wielen; E. J.
Brink; H. F. J. Hendriks; V. N. D.
Taran and I. C. M. Mohede; 2003;
Effects of cis-9, trans-11 And trans-10,
cis-12 Conjugated Linoleic Acid (CLA)
Isomers on Immune Fungtion in
Healthy Men; Eur. L. Clin. Nutr. 57:
– 6003.
Allonso. L; E. P. Guesta and S. E. Gillilang;
; Production of Free Conjugated
Linoleic Acid Isomers by Lactobacillus
acidopphilus casei of Human
Intestinal Origin; J. Dairy Sci 86: 1941 –
Bissonauth P; Y. Chounard; J. Marin; N.
Leblac and H . Jacques; 2008; Altared
Lipids Response in Hamster Fed cis –
trans – 11 and trans – 8, cis - 10
Conjugated Linoleic Acid; Lipids 43
(3): 252 – 258.
Blankson. H; J. A. Stakkestad; H. Fagertun;
E. Thorn; J. Wadstein and O.
Gudmundsen; 2003; Conjugated
Linoleic Acid Reduced Body Fat Mass
in Overweight and Obese Humans. J.
Ntr. 130: 2943 – 2948.
Brahmana. H. R; 1989; Penentuan
Komposisi Asam Lemak Dari Bahan
Alam Dengan Cara Kromatografi Gas
Terhadap Metil Ester Dari Minyak
Nabati (Tidak dipublikasi); Laporan
Penelitian Lemlit USU Medan.
Cristie .W. W ; 1998; Gas Chromatography
–Mass Spectrometry Methods for
Structural Analysis of Fatty Acid;
Lipids 33: 343 – 353.
Cristie .W. W; G. Dopson and R. O. Adlof;
; A Practical Guide to The
Isolation, Analysis and Identification
of Conjugated Linoleic Acid; Lipids 42:
– 1025.
Damyanova B. N; 2010; Silver Ion
Chromatography
and Lipids;
http://www.lipidlibrary.aocs.org/silver/bn
d_rev/idex1.htm
(Accested
on
September 10
th
Dobson. G; 1998; Identification of
Conjugated Linoleic Acid by GC-MS of 4-methyl 1,2,4 – triazoline -3,5 –
Dione Adducts; JAOCS 75: 137 – 142.
Guil - Guerro. J. L; P. Campra Madrid and
E. El – Hassan; 2000; γ - Linoleic Acid
Purification from Seed Oil Sources by
Argentated Silica gel Chromatograpy
Colomn ; J. Chrom. A 694: 381 – 389.
Liangli.Y; 2001; Free Radical Scavenging of
Conjugated Linoleic Acid; J. Agr. and
Food Chem. 49: 3452 – 3456.
Liangli.Y; D. Adams and M. Gabel; 2002,
Conjugated Linoleid Acid Differs in
Their Free Radical Scavenging
Properties; J. Agr. and Food Chem. 50 :
– 4140.
Lin. T. Y; C. W. Lin and Y. J. Wang; 2003;
Production of Conjugated Linoleic
Acid by Enzyme Ekstract of
Lactobacillus Acidophilus CCRC
; Food Chem. 83 : 27 – 31.
Mawarni. R; 2006; Asam Linoleat
Terkonjugasi Penurun Timbunan
Lemak (Tidak dipublikasi);
Laporan Penelitian Pusat Kajian
Makanan Dan Obat Tradisiona Lembaga
Penelitian UNDIP Semarang.
Muller. J. D; and J. E. Delahoy; 2009; CLA
Implications foe Animal Production
and Human Health
:
www.das.psu.edu/temdairy 04 : 1 – 8
(Accested on September 10
th
.
Nagao. K. N; N. Inou; Y. M. Wang and J.
Shimada; 2003; The t10, c12 Isomers of
CLA Suppresses the Development of
Hypertention in Otsuka Long Evans
Tokushima Fatty Rats; Biochem.
Biophys. Res. Comun. 36: 134 – 138.
O’Shea. A. M; R. Devery; F. Lawless; K.
Koegh and T. Stanton; 2000;
Enrichment of The Conjugated
Linoleic Acid Conten in Bovine Milk
by Dry Fractination; Int. Dairy J. 10 :
– 294.
Ozgul. Y. S; 2005; Determination of
Conjugated Linoleic Acid Conten of
Selected Oil Seeds Grown in Turkey;
JAOCS 82 : 893 – 898.
Parodi. P.W; 1997; Conjugated Linoleic
Acid of Milk Fat; J. Dairy Sci. 60:
– 1553.
Pawlosky. R. J; H. W. Sprecher and N.
Salem; 1992; High Sensivity Negative
GC – MS Method for Detection of
Desaturated and chain Elongated
Product of Deuterated Linoleic and
Linolenic Acid; Lipid 33 : 1711 – 1717.
Petridou. M; A. Mougios and M. A. de La
Fuente; 2005; Suplementation with
CLA Isomer Incorporation into Serum
Lipids and Effects on Body Fat
Women; Lipids 38: 805 – 811.
Pilar. L; J. Fontheca; M. Juarez and M. A.
de la Fuente; 2005; Conjugated
Linoleic Acid in Ewe Milk Fat; J. Dairy
Research 72 : 415 – 424.
Priest.W. G and J. D. Von Mikusch;1997;
Composition and Analysis of
Dehydrated Castor Oil; Woburn
Degreasing Company of New Jessey New
York.
Rincon. M. A. C; I. R. Garcia and J. L. GuilGuerro;
; Purification of GLA –
Triglycerides from Evening Primrose
Oil by Gravimetric Colomn
Chromatography; JAOCS 86 : 605 –
Roach. J.A.G; M.P. Yuruwecz; J.G.K.
Kramer; M.M. Mossoba and Y. Ku;
; Gas Chromatography –High
Resolution
Selection Mass
Spectometric Identification of Trace
:0 and 20:2 Fatty Acid Eluting With
Conjugated Linoleic Acid Isomers;
Lipids 35 :797 -802.
Sehat. N; J. K. G. Kramer; M. M. Mossoba;
M. P. Yurawecz; J. A. G. Roach; K.
Eulitz; K. M. Morehouse and Y. Ku;
; Identification of Conjugated
Linoleic Acid Isomers in Cheese by
Gas Chromatography , Silver Ion High
Performance Liquid Chromatography
and Mass Spectral Reconstructed Ion
Profiles
Camparison
of
Chromatographic Elution Sequences;
Lipids 33: 963 - 971.
Sitorus. M; S. Ibrahim; H. Nurdin dan D.
Darwis; 2009; Transformation of
Ricinoleic of Castor Oil into Linoleic
Acid (Omega – 6) and Conjugated
Linoleic Acid by Dehydration; Indo. J.
Chem. 9 (2): 278 – 284.
Sitorus. M; S. Ibrahim; H. Nurdin dan D.
Darwis; 2010; Studi Kinetika Dehidrasi
Risinoleat Minyak Jarak; Jurnal Riset
Kimia; 3 (2): 139 - 144
Sitorus. M; S. Ibrahim; H. Nurdin dan D.
Darwis; 2011
a
; Isomerisasi Linoleat
Minyak jarak Hasil Dehidrasi Menjadi
Asam Linoleat Terkonjugasi dan
Pemisahannya dengan Kromatografi
Kolom Fasa Diam Silikage
Terimpreknasi Perak Nitrat; Jurnal
Matematika dan Sains 16 (1): Inpress
Villeneuve. P; R. Lago; N. Barouh and B.
Barea; 2005; Production of Conjugated
Linoleic Acid Isomers by Dehydration
and Isomerization of Castor Bean Oil;
JAOCS 82 : 261 – 270.
Wisnu.A. Y; 2003; Asam Linoleat
Terkonjugasi Nutrien Ajaib Yang
Sarat Manfaat; Departeman Teknologi
Pangan Universitas Wangsa Manggala
Yogyakarta.
Yuruwecz .M. P and K. M. Morehouse;
; Silver-Ion HPLC of Conjugated
Linoleic Acid Isomers; Eur J Lipid Sci
Technol 103 : 609 -613.
Article Metrics
Abstract view : 270 timesPDF - 1183 times
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.