ISOLATION OF ANTIFUNGI BACTERIA FROM BANANA RHIZOSPHERE TO INHIBIT Fusarium Oxysproum f.sp cubense (FOC) GROWTH

Albert Sembiring, Natalia Lusianingsih Sumanto

Abstract


Fusarium wilt disease on banana has been known as panama disease one of the main diseases that cause huge losses for banana farmers. It is caused by the soil-borne fungal pathogen, Fusarium oxysporum f.sp cubense (Foc), which is very hard control because it is saprophytic in the soil. The mold infiltrates the root to vascular tissue that induces yellowing on the leaf, so this pathogen can attack the root, stem dan leaf. The research aimed to search bacteria from the banana rhizosphere that have an antifungal activity to inhibit Foc growth. Bacteria was isolated by serial dilution then was spread on King’s B agar medium incubation 28oC (room temperature). Four quadrants in vitro test on PDA medium used twenty bacterial from isolation, from the test was obtained six isolates have the potential to inhibit the growth of Foc. Based on percentage inhibition radial growth four isolates that have inhibition 50% over which TR2 was the highest at 79.07%. The in vitro test confirmed that bacteria from the banana rhizosphere have potential as biocontrol agent because it was able to inhibit the Foc growth.


Keywords


Fusarium oxysporum f.sp. cubense, rhizosphere, bacteria, antifungal

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References


Ayyadurai N, Naik PR, M. Rao MS, Kumar RS, Samrat SK, Manohar NM. Sakthivel. 2005. Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana. J App Microbiol. 100:926-937.

Chin-A-Woeng TFC, Bloemberg G V., Mulders IHM, Dekkers LC, Lugtenberg BJJ. 2000. Root Colonization by Phenazine-1-Carboxamide-Producing Bacterium Pseudomonas chlororaphis PCL1391 Is Essential for Biocontrol of Tomato Foot and Root Rot. Mol Plant-Microbe Interact. 13(12):1340-1345.

Ho YN, Chiang HM, Chao CP, Su CC, Hsu HF, Guo CT, Hsieh JL, Huang CC. 2014. In planta biocontrol of soilborne Fusarium wilt of banana through a plant endophytic bacterium, Burkholderia cenocepacia 869T2. Plant soil. 387:295-306.

Huang YH, Wang RC, Lia CH, Zuo CW, Wei YR, Zhang L, Yi GJ. 2012. Control of Fusarium wilt in banana with Chinese leek. Eur J Plant Pathol. 134(1): 87–95.

Kamilova F, Validov S, Azarova T, Mulders I, Lugtenberg B. 2005. Enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria. Environ. Microbiol. 7:1809–17.

Jumidang, Nasir N, Riska, Handayani H. 2005. Teknik Pengujian In Vitro Ketahanan Pisang terhadap Penyakit Layu Fusarium Menggunakan Filtrat Toksin dari Kultur Fusarium oxysporum f. sp. cubense. J Hort. 15(2):135-139

Li S, Jochum CC, Yu F, Zaleta-Rivera K, Du L, Harris SD, Yuen GY. 2008. An antibiotic complex from Lysobacter enzymogenes strain C3: antimicrobial activity and role in plant disease control. Phytopathology. 98:695–701

Nasir N, Jumjunidang, Eliesti F Mehlia Y. 2003. Penyakit layu Panama pada pisang: Observasi ras 4 Fusarium oxysporum f.sp. cubense di Jawa Barat. J. Hort. 13(4):269-275.

Nel B, Steinberg C, Labuschange N, Viljoen A. 2007. Evaluation of fungicides and sterilants for potential application in the management of Fusarium wilt of banana. Crop Protection. 26(4):697–705.

Ploetz, RC, and Pegg, KG. 2000. Fusarium Wilt. hal: 143-159. in: Diseases of banana, abaca and enset. D.R Jones, CABI Publishing. Wallingford UK.

Riastiwi I. 2017. Inventarisasi penyakit tanaman pisang koleksi kebun plasma nuftah, cibinong science center-BG. Jurnal Mikologi Indonesia. 1(1):38-44.

Semangun, H. 2007. Penyakit-Penyakit Tanaman Hortikultura di Indonesia. Gadjah Mada University Press, Yogyakarta.

Sukmadjaja, D., R. Purnamaningsih, Y. Supriati, dan S. Rahayu. 2010. Rekayasa genetik dan mutasi pisang varietas Ambon Kuning untuk memperoleh pisang dengan produktivitas 15 ton/ha dan tahan 60% terhadap penyakit Fusarium. Laporan Hasil Penelitian Program Riset Insentif 2010. Balai Besar Litbang Bioteknologi dan Sumberdaya Genetik Pertanian, Badan Litbang Pertanian. 21 hlm

Sumardiyono C, Suharyanto S, Suryanti S, Rositasari P, Chinta YF. 2016. Deteksi pengimbasan ketahanan pisang terhadap penyakit layu fusarium dengan asam fusarat. J Perlin Tan Indo. 19(1):40-44.

Tan, Z, Lin B, Zhang R. 2013. A novel antifungal protein of Bacillus subtilis B25. Springerplus. 2:543.

Van Loon LC. 2007. Plant responses to plant growth-promoting bacteria. Eur. J. Plant Pathol. 119:243–54.

Weller DM, and Thomashow LS. 2007. Molecular Ecology of Rhizosphere Microorganisms. Current Challenges in Introducing Beneficial Microorganisms into the Rhizosphere.

Xue, C, Penton, CR, Shen Z, Zhang R, Huang Q, Li R, Ruan Y, Shen Q. 2015. Manipulating the banana rhizosphere microbiome for biological control of Panama disease. Sci Rep. 5:1-11.




DOI: https://doi.org/10.24114/jbio.v5i3.15293

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JBIO : Jurnal Biosains (The Journal of Biosciences), Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan. ISSN 2443-1230 (print) dan ISSN 2460-6804 (online)

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