METABOLITE ACTIVITY OF LACTIC ACID BACTERIA FROM SUMBAWA HORSE MILK, WEST NUSA TENGGARA, INDONESIA

Kusdianawati Kusdianawati, Apon Zaenal Mustopa, Fatimah Fatimah, Nurlaili Ekawati

Abstract


Sumbawa horse milk is one of the local products in Sumbawa (West Nusa Tenggara). There is no research so far which discusses the potencies of LAB metabolite compounds as antimicrobials and probiotics for treating Diabetes Mellitus type 2. This study aims to obtain the antimicrobial and inhibitory activity of the LAB-extracted metabolite compounds from Sumbawa horse milk collected from Penyaring village and Lennanguar village, Sumbawa, West Nusa Tenggara, Indonesia. LAB metabolites are extracted by using 96% ethanol. It has been found that metabolites extracted from media culture of LAB SKP K.9 (E. thailandicus) have the highest antimicrobial activity against S. aureus (ATCC 6538) (2.5 cm), S. typhimurium (2.5 cm), L. monocytogenes (BTCC B693) (2.53 cm) and EPEC K.1.1 (2.7 cm) when compared with other LAB isolates. Moreover, the inhibition of α-glucosidase enzyme obtained from LAB SKP K.5 isolate (L. garvieae) is 6.25%, 39.28% from LAB SKP K.9 (E. thailandicus), 53.57% from LAB SKP K.3 (W. confusa), 67.85% from LAB SKL K.4 (L. fermentum), and 78.57% from LAB SK 1.5 (E. faecium). The extract of LAB SK 1.5 metabolite compound (E. faecium) has the highest inhibitory activity against the α-glucosidase enzyme (78.57%) compared to other LAB isolates.


Keywords


antimicrobial; lactic acid bacteria (LAB); Sumbawa horse milk; α-glucosidase enzyme

Full Text:

PDF

References


Abdelfatah EN, Mahboub HHH. 2018. Studies on the effect of Lactococcus garvieae of dairy origin on both cheese and Nile tilapia (O. niloticus). Int J Vet Sci Med. 6:201–207. doi:10.1016/j.ijvsm.2018.11.002.

Arief I, Jakaria, Suryati T, Wulandari Z, Andreas E. 2013. Isolation and characterization of plantaricin produced by Lactobacillus plantarum strains (IIA-1A5, IIA-1B1, IIA-2B2). Media Patern. 36(2):91–100. doi:10.5398/medpet.2-13.36.2.91.

Azad M, Sarker M, Li T, Yin J. 2018. Probiotic species in the modulation of gut microbiota: an overview. Biomed Res Int. doi:10.1155/2018/9478630.

Baipai V, Han J, Nam G, Majumder R, Park C, Lim J, Paek W, Rather I, Park Y. 2016. Characterization and pharmacological potential of Lactobacillus sakei 1I1 isolated from fresh water fish Zacco koreanus. 24: 1-12. J Pharm Sci Res. 24:1–12. doi:10.1186/s40199-016-0147-8.

Chen P, Zhang Q, Dang H, Liu X, Tian F, Zhao J, Chen Y, Zhang H, Chan W. 2014. Screening for potential new probiotic based on probiotic properties and α- glucosidase inhibitory activity. Food Control. 35:65–72.

Da-Silva S, Michele V, Jose M, Ricardo P. 2014. Overview of Lactobacillus plantarum as a promising bacteriocin producer among lactic acid bacteria. Food Res Int. 64:527–536.

Elmoslih A, Zanzan M, Aissa R. 2017. Isolation and characterization of bacteriocinogenic enterococcal and lactococcal strains from south of Morocco dairy product. Biotechnol J Int. 18(4):1–16.

Farida E, Jenie B, Nuraida L, Giriwono P. 2019. Aktivitas antioksidan dan penghambatan α-glukosidase oleh ekstrak etanol bakteri asam laktat indigenus. J Teknol dan Ind Pangan. 30(1):56–63. doi:10.6066/jtip.2019.30.1.56.

Georgieva R, Yocheva L, Tserovska L, Zhelezova G, Stefanova N, Atanasova A, Danguleva A, Ivanova G, Karapetkov N, Rumyan N, et al. 2015. Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Biotechnol Equip. 29(1):84–91. doi:10.1080/13102818.2014.987450.

Goh H, Philip K. 2015. Purification and characterization of bacteriocin produced by Weissella confusa A3 of dairy origin. PLoS One. 10:10. doi:10.1371/journal.pone.0140434.

Hadji-Sfaxi I, El-Ghaish S, Ahmadova A, Batdorj B, Gwenaëlle Le Blay-Laliberté G, Barbier G, Haertlé T, Jean-Marc C. 2011. Antimicrobial activity and safety of use of Enterococcus faecium PC4.1 isolated from Mongol yogurt. Food Control Vol. 22(12):2020–2027. doi:https://doi.org/10.1016/j.foodcont.2011.05.023.

Hakim N, Suada I, Sampurna I. 2013. Ketahanan susu kuda Sumbawa pada penyimpanan suhu ruang ditinjau dari total asam, uji didih dan warna. Indones Med Veterinus. 2(4):369–374.

Hanchi H, Mottawae W, Sebei K, Hammami R. 2018. The genus Enterococcus: between probiotic potential and safety concerns-an update. Front Microbiol. 9:1791.

He M, Shi B. 2017. Gut microbiota as a potential target of metabolic syndrome: the role of probiotics and prebiotics. Cell Biosci. 7(1):54. doi:10.1186/s13578-017-0183-1.

Hermawati D. 2005. Kajian aktivitas dan karakterisasi senyawa antimikroba dari susu kuda Sumbawa [Disertasi]. [Bogor] : Institut Pertanian Bogor.

Jong-Su P, Shin E, Hong H, Hyun-Jung S, Young-Hoon C, Ki-Hyun A, Paek K, Lee Y. 2015. Characterization of Lactobacillus fermentum PL9988 isolated from healthy elderly Korean in a longevity village. J Microbiol Biotechnol. 25(9):1510–1518. doi:10.4014/jmb.1505.0501.

Karimae S, Sadeghi J, Asadian M, Esghaei M, Pourshafie M, Talebi M. 2016. Antibacterial potential and genetic profile of Enterococcus faecium strains isolated from human normal flora. Microb Pathog. 96:67-71. doi:10.1016/j.micpath.2016.05.004.

Kusdianawati, Mustopa A, Suharsono, Budiarto B, Fatimah, Danuri H. 2015. Construction, expression and purification of recombinant pre-mature peptide of plantaricin F from Lactobacillus plantarum S34 in Escherichia coli. Indones J Agr Sci. 16(1):31–38.

Kusdianawati, Mustopa A, Fatimah, Budiarto B. 2020. Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia. Biodiv. 21(7):3225-3233. doi:10.13057/biodiv/d210744.

Lee T, Pang S, Abraham S, Coombs G. 2019. Review antimicrobial-resistant CC17 Enterococcus faecium: the past, the present and the future. J Glob Antimicrob Resist. 16:36–47. doi:10.1016/j.jgar.2018.08.016.

Lopetuso L, Giorgio M, Saviano A, Scaldaferri F, Gasbarrini A, Cammarota G. 2019. Bacteriocins and bacteriophages: therapeutic weapons for gastrointestinal diseases? Int J Mol Sci. 20(1):183. doi:10.3390/ijms20010183.

Mayo B, Aleksandrzak T, Fernández M, Kowalczyk M, Álvarez P, Bardowski J. 2010. Biotechnology of lactic acid bacteria: Novel Applications. Iowa (US): Wiley-Blackwell.

Morandi S, Silvetti T, Brasca M. 2013. Biotechnological and safety characterization of Enterococcus lactis, a recently described species of dairy origin. Antonie van Leeuwenhoek-Journal Microbiol. 103(1):239–249.

Mustopa A, LABia R, Putranto W, Ridwan M, Solehudin M. 2010. Penapisan bakteri asam laktat yang diisolasi dari bekasam daging sapi dalam menghasilkan bakteriosin untuk menghambat bakteri patogen. In: Seminar Nasional Fakultas Peternakan UNPAD ke-2, Sistem Produksi Berbasis Ekosistem Lokal. Bandung: Universitas Padjadjaran.

Mustopa A, Kusdianawati, Fatimah, Umami R, Budiarto R, Danuri H. 2016. Cloning and expression of plantaricin E and F genes of Lactobacillus plantarum S34 isolated from Indonesia traditional-fermented meat (Bekasam). Int Food Res J. 23(2):762–769.

Mustopa A, Mariyah S, Fatimah, Budiarti S, Murtiyaningsih W, Alfisyahrin W. 2018. Construction, heterologous expression, partial purification, and in vitro cytotoxicity of the recombinant plantaricin E produced by Lactococcus lactis against Enteropathogenic Escherichia coli K.1.1 and human cervical carcinoma (HeLa) cells. Mol Biol Rep. 45:1235–1244. doi:10.1007/s11033-018-4277-6.

Nurhayati R, Miftakhussolikhah, Andri F, Desy L. 2017. Lactic acid bacteria producing inhibitor of alpha glucosidase isolated from ganyong (Canna edulis) and kimpul (Xanthosoma sagittifolium). In: Earth and Environmental Science 101.

Panwar H, Calderwood D, Grant I, Grover S, Green B. 2014. Lactobacillus strains isolated from infant faeces possess potent inhibitory activity against intestinal alpha- and beta-glucosidases suggesting anti-diabetic potential. Eur J Nutr. 53(7):1465–1474. doi:10.1007/s00394-013-0649-9.

Sales P, Souza P, Simeoni L, Magalhaes P, Silveira D. 2012. α-amylase inhibitor: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci. 15:141–183. doi:10.18433/J35S3K.

Sangwan S, Singh R. 2014. Therapeutic effects of probiotic fermented milk (LGG and L. casei NCDC 19) on progression of type 2 diabetes. J Innov Biol. 1(2):78–83.

Sari P, Nurliana, Hasan M, Sayuti A, Sugito A. 2017. Lactobacillus casei fermented milk as a treatment for diabetes in mice (Mus musculus). J Med Vet. 11(1):15–19. doi:10.21157/j.med.vet.v11i1.4065.

Shah N, Patel A, AmLABam P, Holst O, Ljungh A, Prajapati J. 2016. Determination of an antimicrobial activity of Weissella confusa, Lactobacillus fermentum, and Lactobacillus plantarum against clinical pathogenic strains of Escherichia coli and Staphylococcus aureus in co-culture. Ann Microbiol. 66:1137. doi:10.1007/s13213-016-1201-y.

da Silva do Nascimento M, Moreno I, Kuaye A. 2010. Antimicrobial activity of Enterococcus Faecium Fair-E 198 against Gram-positive pathogens. Braz J Microbiol. 41(1):74–81. doi:10.1590/S1517838220100001000013.

Sujaya N, Ramona Y, Widarini N, Suariani N, Dwipayanti N. 2008. Isolasi dan karakterisasi bakteri asam laktat dari susu kuda Sumbawa. J Vet. 9(2):52–59.

Suneel D, Basappa K. 2013. Identification and characterization of Lactococcus garvieae and antimicrobial activity of its bacteriocin isolated from cow’s milk. Asian J Pharm Clin Res. 6(Suppl 3):104–108.

Tenea G, Lara M. 2019. Antimicrobial compounds produced by Weissella confusa cys2-2 strain inhibit Gram-negative bacteria growth. CyTA, J Food. 17:105–111. doi:10.1080/19476337.2018.1561520.

Vieco-Saiz N, Belguesmia Y, Raspoet R, Auclair E, Gancel F, Kempf I, Drider D. 2019. Benefits and inputs from lactic acid bacteria and their bacteriocins as alternatives to antibiotic growth promoters during food-animal production. Front Microbiol. 10:57. doi:10.3389/fmicb.2019.00057.

Yahya R. 2012. Karakteristik mikrobiologis dan aktivitas antimikroba susu kuda fermentasi koumiss terhadap Salmonella typhirium dan Mycobacterium tuberculosis [Skripsi]. [Bogor] : Institut Pertanian Bogor.

Ye K, Liu J, Liu M, Huang Y, Wang K, Zhou G. 2018. Effects of two Weissella viridescens strains on Listeria monocytogenes grow that different initial inoculum proportions. CyTA-Journal Food. 16(1):299–305.

Yun S, Park H, Kang J. 2009. Effect of Lactobacillus gasseri BNR17 on blood glucose levels and body weight in a mouse model of type 2 diabetes. J Appl Microbiol. 107(5):1681–168.

Zacharof MP, Lovitt RW. 2012. Bacteriocins Produced by Lactic Acid Bacteria a Review Article. In: APCBEE Procedia. Vol. 2. Elsevier B.V. p. 50–56. http://dx.doi.org/10.1016/j.apcbee.2012.06.010.




DOI: https://doi.org/10.24114/jbio.v9i1.37701

Article Metrics

Abstract view : 158 times
PDF - 113 times

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

INDEXING

  

 

 

 

JBIO : Jurnal Biosains (The Journal of Biosciences), Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan. ISSN 2443-1230 (print) dan ISSN 2460-6804 (online)

Lisensi Creative Commons
Ciptaan disebarluaskan di bawah Lisensi Creative Commons Atribusi 4.0 Internasional.