Comparison Cytological Staining Results of Pleural Effusion with Delayed Time Without Alcohol Fixative and With Alcohol Fixative
PERBANDINGAN HASIL PEWARNAAN SITOLOGI EFUSI PLEURA DENGAN WAKTU TUNDA TANPA FIKSATIF ALKOHOL DAN DENGAN FIKSATIF ALKOHOL
DOI:
https://doi.org/10.24114/jbio.v11i3.68026Abstract
The delay in the examination of pleural effusion fluid may lead to damage to the specimens. To minimize this damage, the addition of fixative solutions is often employed, one of which is 50% alcohol. Therefore, this study aims to determine the effectiveness of using 50% alcohol in fixing smears of pleural effusion fluid that are delayed in examination, as well as to assess the morphological quality of cells in effusion smears stored with and without fixative solutions. The research method is experimental, utilizing a pretest-posttest design. Sampling was conducted using accidental sampling, with specimens categorized into four treatment groups (immediate examination, immediate examination with 50% alcohol fixative, delayed examination without fixative, and delayed examination with 50% alcohol fixative). Smears were stained using the Diff-Quik method. The assessment results were analyzed descriptively and with a paired T-test. In the comparison between immediate without and delayed without, the T-test results showed a significant difference (p = 0.002, <0.05). Conversely, in the comparison between immediate and delayed with fixative, no significant difference was found (p = 0.707, >0.05). The comparison between immediate without and immediate with fixative showed a significant change (p = 0.005, <0.05), while the comparison between delayed without fixative and delayed with fixative was not significant (p = 0.775, >0.05). The alternative hypothesis was rejected based on the paired T-test, as no significant difference was found in the delayed samples compared to the samples that were delayed with the addition of 50% alcohol fixative.References
Anita, Hervani Ayu, Budi Santosa, and Husni Zulfikar Farug. 2018. Effect of Variation in Delay in Administration of Fixation Solution of Peripheral Blood Smear Preparations on Erythrocyte Morphology. Semarang.
Astuti, Deni Indri. 2017. "Microscopic Quality Picture in Clinically Diagnosed FNAB Samples of Suspected Mammae Carcinoma by Diff Quick and Papanicolau Painting Methods." University of Muhammadiyah Semarang, Semarang.
Dila, Tiara Rahma. 2023. Comparison of Giemsa, Diff Quick, and Papanicolau Dyes of Pleural Effusion Cytology Preparations in Outpatient and Inpatient at A. W Sjahranie Samarinda Hospital. Samarinda.
Erick, Khristian, and Inderiati Dewi. 2017. Cytostotechnology. https://poltekkesbanten.ac.id/wp-content/uploads/2017/12/Sitohistoteknologi-SC.pdf.
Hayuningrum, Dima Fitri. 2020. "Diagnosis of Pleural Effusion." Journal of Professional Nursing Research 2 No.4:529–36. http://jurnal.globalhealthsciencegroup.com/index.php/JPPP.
Inderiati, Dewi, and Bella Eka Pratiwi. 2021. "Comparison of NAFS Fixative Solution with 96%-10% NBF Alcohol in Cell Block Manufacturing in Pleural Fluid Samples." JoIMedLabS 1(1):39–55.
Kopcinovic, Lara Milevoj, and Jelena Culej. 2021. “Preanalytical Phase in Pleural Fluid Analysis.” Jurnal Of Laboratory and Precision Medicine 6. htppa;//jlpm.amegroups.org/article/view/6322/html.
Mizan, Muhammad Naufal, Maya Damayanti, and Fitri Nuroini. n.d. 2021. The The Descriptions Of Oral Cavity On Mucous Epithelial Cytology Staining Hibiscus Flower Extract (Hibiscus Rosa-Sinensis L.).
Nuryadi, Tutut Dewi Astuti, Endang Sri Untami, and M. Budiantara. 2017. Fundamentals of Research Statistics. Yogyakarta: Sibuku Media.
Oktaviana, Claudia Ayu Aulia. 2021. Nursing Care for Mrs. L with Medical Diagnosis of Pleural Effusion in the Emergency Room of Rumkital Dr. Ramelan Surabaya 2021. Surabaya.
Prasetyani, period. 2017. Histological Microscopic Picture of Pleural Effusion Block Using 70% Alcohol and 10% BNF Fixation on HE Staining. Semarang.
Princess, Dinda. 2022. Analysis of the Quality of Papanicolau Staining in Smear Preparations and Pleural Effusion Cell Blocks : A Literature Review.
daughter, Rachma Greta Pradana. 2022. Anatomical Pathology Practicum Module Book Block 2.1 2022. Yogyakarta: Faculty of Medicine, Ahmad Dahlan University.
Raymond, Nora. 2022. "Handling of Anatomical Pathological Tissues." https://yankes.kemkes.go.id/view_artikel/577/penanganan-jaringan-patologi-anatomi.
Rinaldi, Sony Faisal, and Bagya Mujianto. 2017. Research Methodology and Statistics. Vol. 1. 2017th ed. Center for Health Human Resources Education, Agency for Development and Empowerment of Human Resources Healtha.
Saldi, Rifky, A. Wahid, and Andi Jumriati. 2022. Handling of Transudate and Exudate Pleural Fluid from Pleural Effusion Patients. Vol. 2022.
Sudirman, Suri Tuding Lembang, Marilyn Lasharus Kondolayuk, et al. 2023. Education Statistics. edited by S. Haryanti. Bandung: CV. INDONESIAN SCIENCE MEDIA.
Syafaat, Mohamad, and Wulan Fitriani Safari. 2024. "Design and Build Automatic Volumetric Filling Equipment as an Effort to Ensure Quality Assurance of Clinical Laboratory Examination Results." Journal of Technology 11(2):2024–2160. doi:10.31479/jtek.v11i2.300.
Tarigan, Eva Sri Ayu. 2023. “Description Of The Results Of The Cytological Examination Of Pleural Fluid Using Giemsa Staining.” MEDISTRA MEDICAL JOURNAL (MMJ) 1(1):7–12. doi:10.35451/mmj.v1i1.1944.
Vinod B Shidam. 2022. “Collection and Processing of Effusion Fluids For Cytopathologic Evaluation.” CytoJournal. doi:10.25259
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