Nutrient Evaluation of an Enteral formula Compounded from Soya Bean (Glycine max), Finger Millet (Eleusine coracana), and Red Sorghum (Sorghum bicolor)
DOI:
https://doi.org/10.24114/hhzw8920Keywords:
Enteral nutrition, hospital malnutrition, nutrient-dense crops, sustainable therapeutic feeds.Abstract
The high cost and limited availability of commercial enteral formulas in resource-poor settings like Nigeria necessitate the development of nutritious, affordable, and locally-sourced alternatives. Soybean, finger millet, and red sorghum are underutilized and locally available crops with exceptional nutritional profiles suitable for compounding enteral feeds for clinical nutrition. This study aimed to formulate and evaluate the nutrient composition of enteral feed powders compounded from blends of soybean, finger millet, and red sorghum. Mixed samples of soybean, finger millet, and red sorghum were purchased from the different markets in Cross River State. Nigeria and processed using various local methods (fermentation, roasting, and sprouting). The processed flours were combined in 30: 70 ratios to create distinct compounded formulations. The proximate composition (moisture, ash, crude protein, fat, dietary fibre, and carbohydrates) and mineral content (Mg, Fe, Ca, Zn, K, Mn, etc.) were determined using standard AOAC methods and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Data were analyzed using SPSS version 26. Results showed that soybean-based formulations were significantly higher in protein (28.68–34.82%), fat (16.64–23.58%), and dietary fibre (19.01–23.13%), while cereal-based formulations recorded higher carbohydrates (65.74–74.06%). Sprouting significantly enhanced the mineral density of the cereals, particularly calcium in finger millet (406.69 mg/100g) and iron in red sorghum (6.16 mg/100g). The final blended formulations (F1–F6) showed balanced profiles, with protein content ranging from 14.66% to 16.01% and enhanced mineral levels, especially in formulations containing sprouted ingredients. Blending soybean, finger millet, and red sorghum can produce nutritionally rich, cost-effective, and locally sustainable enteral feed powders. These formulations showed a viable alternative to imported commercial products, potentially improving nutrition support in Nigerian hospitals. Future research should focus on bioavailability, sensory evaluation, and shelf-life stability.
References
AACC International. (2010). Approved Methods of Analysis (11th ed.). AACC International. Retrieved from https://www.aaccnet.org/publications/methods
Abioye, V., Olatunde, S., Ogunlakin, G., & Abioye, O. A. (2022). Effect of soaking conditions on chemical composition, antioxidant activity, total phenols, flavonoids and anti-nutritional contents of finger millet. African Journal of Food, Agriculture, Nutrition and Development, 22(8), 20210–20228. https://doi.org/10.18697/ajfand.112.20480
AOAC International. (2019). Official Methods of Analysis of AOAC INTERNATIONAL (21st ed.). AOAC International. Retrieved from https://members.aoac.org/AOAC/AOAC/Item_Detail.aspx?iProductCode=1121&Category=OMA
Asrani, P., Ali, A., & Tiwari, K. (2023). Millets as an alternative diet for gluten-sensitive individuals: A critical review on nutritional components, sensitivities and popularity of wheat and millets among consumers. Food Reviews International, 39(6), 3370–3399. https://doi.org/10.1080/87559129.2021.2012790
Awika, J. M. (2017). Sorghum: Its unique nutritional and health-promoting attributes. In Gluten-Free Ancient Grains (pp. 21-54). Woodhead Publishing. https://doi.org/10.1016/b978-0-08-100866-9.00003-0
Bechtold, M. L., Brown, P. M., Escuro, A., Grenda, B., Johnston, T., Kozeniecki, M., & ASPEN Enteral Nutrition Committee. (2022). When is enteral nutrition indicated? Journal of Parenteral and Enteral Nutrition, 46(7), 1470–1496. https://doi.org/10.1002/jpen.2364
Cederholm, T., Barazzoni, R. O. C. C. O., Austin, P., Ballmer, P., Biolo, G. I. A. N. N. I., Bischoff, S. C., & Singer, P. (2017). ESPEN guidelines on definitions and terminology of clinical nutrition. Clinical nutrition, 36(1), 49-64. https://doi.org/10.1016/j.clnu.2016.09.004
Chauhan, D., Kumar, K., Ahmed, N., Thakur, P., Rizvi, Q., Jan, S., & Yadav, A. N. (2022). Impact of soaking, germination, fermentation, and roasting treatments on nutritional, anti-nutritional, and bioactive composition of black soybean (Glycine max L.). Journal of Applied Biology & Biotechnology, 10(4), 1–9. https://doi.org/10.7324/jabb.2022.100523
Davana, T. V., Revanna, M., & Begum, S. (2021). Effect of Malting on the Nutritional Composition, Anti-nutrition Factors and Mineral Composition on Sorghum (Sorghum bicolor). Asian Journal of Dairy and Food Research, 40(3), 234–240. https://doi.org/10.18805/ajdfr.dr-1624
Dey, B. B., Choudhury, M., & Das, M. (2025). Impacts of Soaking Conditions on Proximate Composition of Finger Millet. Journal of Advances in Biology & Biotechnology, 28(1), 1–10. https://doi.org/10.9734/jabb/2025/v28i72555
Dhliwayo, T., Chopera, P., Matsungo, T., Chidewe, C., Mukanganyama, S., Nyakudya, E., Mtambanengwe, F., Mapfumo, P., & Nyanga, L. K. (2023). Effect of germination and roasting on the proximate, mineral and anti-nutritional factors in finger millet (Eleucine Coracana), Cowpeas (Vigna Unguiculata) and orange maize (Zea mays). African Journal of Food, Agriculture, Nutrition and Development, 23(3), 21567–21585. https://doi.org/10.18697/ajfand.123.22960
Doley, J. (2022). Enteral nutrition overview. Nutrients, 14(11), 2180.
Elliott, H., Woods, P., Green, B., & Nugent, A. (2022). Can sprouting reduce phytate and improve the nutritional composition and nutrient bioaccessibility in cereals and legumes? Nutrition Bulletin, 47(2), 202–215. https://doi.org/10.1111/nbu.12549
Ha, M.-C., Im, D.-Y., Park, H.-S., Dhungana, S. K., Kim, I.-D., & Shin, D.-H. (2022). Seed Treatment with Illite Enhanced Yield and Nutritional Value of Soybean Sprouts. Molecules, 27(3), 1002. https://doi.org/10.3390/molecules27041152
Holodová, M., Čobanová, K., Šefčíková, Z., Barszcz, M., Tuśnio, A., Taciak, M., & Grešáková, Ľ. (2019). Dietary Zinc and Fibre Source can Influence the Mineral and Antioxidant Status of Piglets. Animals : an Open Access Journal from MDPI, 9. https://doi.org/10.3390/ani9080497.
Iyabo, O. O., Ibiyinka, O., & Deola, O. A. (2020). Comparative study of nutritional, functional and antinutritional properties of white sorghum bicolor (sorghum) and pennisetum glaucum (pearl millet). International Journal of Engineering Technologies and Management Research, 7(2), 1–10. https://doi.org/10.29121/ijetmr.v5.i3.2018.187
Jindal, A., Patil, N., Bains, A., Sridhar, K., Stephen Inbaraj, B., Tripathi, M., ... & Sharma, M. (2023). Recent trends in cereal-and legume-based protein-mineral complexes: formulation methods, toxicity, and food applications. Foods, 12(21), 3898. https://doi.org/10.3390/foods12213898
Kamau, E., Mutungi, C., Mutungi, C., Kinyuru, J., Imathiu, S., Tanga, C., Affognon, H., Ekesi, S., Nakimbugwe, D., & Fiaboe, K. (2018). Moisture adsorption properties and shelf-life estimation of dried and pulverised edible house cricket Acheta domesticus (L.) and black soldier fly larvae Hermetia illucens (L.). Food research international, 106, 420-427. https://doi.org/10.1016/j.foodres.2018.01.012. https://doi.org/10.1016/j.foodres.2018.01.012
Kayisoglu, C., Altıkardeş, E., Guzel, N., & Uzel, S. (2024). Germination: A Powerful Way to Improve the Nutritional, Functional, and Molecular Properties of White- and Red-Colored Sorghum Grains. Foods, 13(2), 234. https://doi.org/10.3390/foods13050662
Keyata, E. O., Tola, Y., Bultosa, G., & Forsido, S. (2021). Premilling treatments effects on nutritional composition, antinutritional factors, and in vitro mineral bioavailability of the improved Assosa I sorghum variety (Sorghum bicolor L.). Food Science & Nutrition, 9(2), 1102–1112. https://doi.org/10.1002/fsn3.2155
Kumar, A., Kaur, A., Gupta, K., Gat, Y., & Kumar, V. (2021). Assessment Of Germination Time of Finger Millet for Value Addition in Functional Foods. Current Science, 120(2), 234–241. https://doi.org/10.18520/cs/v120/i2/406-413
Munguti, J., Muthoka, M., Chepkirui, M., Kyule, D., Obiero, K., Ogello, E., … & Kwikiriza, G. (2024). The fish feed sector in Kenya, Uganda, Tanzania, and Rwanda: Current status, challenges, and strategies for improvement—A comprehensive review. Aquaculture Nutrition, 2024(1), 8484451. https://doi.org/10.1155/2024/8484451
Nkhata, S. G., Ayua, E., Kamau, E. H., & Shingiro, J. (2018). Fermentation and germination improve nutritional value of cereals and legumes through activation of endogenous enzymes. Food Science & Nutrition, 6(8), 2446–2458. https://doi.org/10.1002/fsn3.846
Ouédraogo, E. R., Konaté, K., Dakuyo, R., Kaboré, K., Sanou, A., Sama, H., & Dicko, M. H. (2022). Optimization of soybean pre-treatment processes for the improvement of their nutritional, biochemical and bioactive characteristics. World Journal of Advanced Research and Reviews, 15(2), 1–12.S. https://doi.org/10.30574/wjarr.2022.15.2.0700
Patil, P., Singh, S. P., & Patel, P. (2023). Functional properties and health benefits of finger millet (Eleusine coracana L.): A review. The Journal of Phytopharmacology, 12(3), 196–202. https://doi.org/10.31254/phyto.2023.12308
Pierzak, M., Szczukiewicz-Markowska, G., & Głuszek, S. (2020). The problem of hospital malnutrition and its consequences. Medical Studies/Studia Medyczne, 36(1), 46–50. https://doi.org/10.5114/ms.2020.94088
Putri, T. P., Mitra, M., & Devi, L. S. (2025). Analysis of Commercial Enteral Formula (CEF) and Hospital Enteral Formula (HEF) usage on cost budget and patient food acceptance. Majalah Kesehatan Indonesia, 6(1), 9-16. https://doi.org/10.47679/makein.2025221
Rousseau, S., Kyomugasho, C., Celus, M., Hendrickx, M., & Grauwet, T. (2020). Barriers impairing mineral bioaccessibility and bioavailability in plant-based foods and the perspectives for food processing. Critical Reviews in Food Science and Nutrition, 60(10), 1622–1635. https://doi.org/10.1080/10408398.2018.1552243
Sheethal, H. V., Baruah, C., Subhash, K., Ananthan, R., & Longvah, T. (2022). Insights of Nutritional and Anti-nutritional Retention in Traditionally Processed Millets. Frontiers in Nutrition, 9, 834567. https://doi.org/10.3389/fsufs.2021.735356
Siddique, K. H., Johansen, C., Turner, N. C., Jeuffroy, M. H., Hashem, A., Sakar, D., ... & Alghamdi, S. S. (2012). Innovations in agronomy for food legumes. A review. Agronomy for sustainable development, 32(1), 45-64. https://doi.org/10.1007/s13593-011-0021-5
Singh, A., Sharma, S., & Singh, B. (2017). Effect of germination time and temperature on the functionality and protein solubility of sorghum flour. Journal of Cereal Science, 76, 131-139. https://doi.org/10.1016/j.jcs.2017.06.003
Temba, M., Njobeh, P., Adebo, O., Olugbile, A., & Kayitesi, E. (2016). The role of compositing cereals with legumes to alleviate protein energy malnutrition in Africa. International Journal of Food Science and Technology, 51, 543-554. https://doi.org/10.1111/ijfs.13035.
Zambrano, M., Dutta, B., Mercer, D., MacLean, H., & Touchie, M. (2019). Assessment of moisture content measurement methods of dried food products in small-scale operations in developing countries: A review. Trends in Food Science & Technology. https://doi.org/10.1016/j.tifs.2019.04.006
Zewudie, K., & Gemede, H. (2024). Assessment of nutritional, antinutritional, antioxidant and functional properties of different soybean varieties: implications for soy milk development. Cogent Food & Agriculture, 10. https://doi.org/10.1080/23311932.2024.2380496
Zhang, J., Li, W., Ying, Z., Zhao, D., Yi, G., Li, H., & Liu, X. (2020). Soybean protein-derived peptide nutriment increases negative nitrogen balance in burn injury-induced inflammatory stress response in aged rats through the modulation of white blood cells and immune factors. Food & Nutrition Research, 64. https://doi.org/10.29219/fnr.v64.3677
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