PENGARUH EKSTRAK ETANOL Plectranthus amboinicus Lour Spreng TERHADAP BERAT BADAN DAN BERAT RELATIF ORGAN TIKUS YANG DINDUKSI KANKER KULIT DENGAN DMBA

Melva Silitonga, Erlintan Sinaga, Pasar Maulim Silitonga

Abstract


Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun Plectranthus amboinicus terhadap  berat dan berat relatif organ dan berat badan tikus yang diinduksi kanker kulit dengan DMBA. Digunakan 24 ekor tikus dan dibagi menjadi empat kelompok yaitu K-, K+, T1 dan T2. K- yaitu kelompok kontrol diberi CMC 0.5%. K+  diberi DMBA 175 µg, T1 dan T2 diberi DMBA 175 µg dan EEP sebanyak 250 dan 500 mg/kg berat badan. Induksi DMBA diterapkan secara topical dibagian sisi posterior punggung dengan luas 3×3cm. DMBA dilarutkan dalam 0.1 ml aceton dioleskan. seminggu dua kali selama delapan minggu. Pada minggu ke sembilan, diberikan EEP setiap hari selama empat minggu pada kelompok T1 dan T2. Selama penelitian semua tikus diberi pakan dan minum secara ad libitum. Pada minggu ke 13 tikus dibunuh lalu dibedah untuk memperoleh organ ginjal, paru, hati dan limpa. Data yang diperoleh dianalisis dengan Anova. Hasil penelitian menunjukkan induksi kanker kulit dengan DMBA meningkatkan berat jantung, paru dan ginjal. Pemberian EEP menurunkan berat organ tersebut  menjadi sama dengan kontrol. DMBA meningkatkan berat relatif organ jantung, ginjal, paru-paru dan hati. EEP menurunkan berat relatif organ.  Pemberian DMBA menurunkan berat  badan mingguan pada tikus perlakuak K+, T1 dan T2. Pemberian EEP meningkatkan berat badan tikus yang diinduksi kanker kulit dengan DMBA


Keywords


Plectranthus amboinicus, berat relative organ, berat badan, DMBA

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References


Alvin Jose, M., Ibrahim, & Janardhanan, S. (2005). Modulatory effect ofPlectranthus amboinicusLour. on ethylene glycol-induced nephrolithiasis in rats. Indian Journal of Pharmacology, 37(1), 43. doi:10.4103/0253-7613.13857

Arora, R., Bhushan, S., Kumar, R., Mannan, R., Kaur, P., Singh, A. P., … Arora, S. (2014). Hepatic Dysfunction Induced by 7, 12-Dimethylbenz(α)anthracene and Its Obviation with Erucin Using Enzymatic and Histological Changes as Indicators. PLoS ONE, 9(11), e112614. doi:10.1371/journal.pone.0112614

Arumugam, G., Swamy, M., & Sinniah, U. (2016). Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules, 21(4), 369. doi:10.3390/molecules21040369

Amarasiri, A., Attanayake, AP., Jayatilaka KAPW and LKB Mudduwa (2018). Acute nephroprotective and antioxidant activities of aqueous leaf extract of Plectranthus amboinicus (Roxb.) grown in Sri Lanka Journal of Pharmacognosy and Phytochemistry 2018; 7(4): 155-161

Ashrafi, M., Karimi, B., Sabahi, M., & Shomali, T. (2018). Hepatoprotective effect of simvastatin in mice with DMBA-induced breast cancer: Histopathological, biochemical and antioxidant status evaluation. Biomedical Research and Therapy, 5(3), 2064–2077. doi:10.15419/bmrat.v5i3.421

Asiimwe, S, Anna-Karin B., Azeem, M., Kamatenesi Maud Mugisha., Agnes Namutibe dan Ndukui James Gakunga.2014.Chemical Composition and Toxicological Evaluation of The Aqueous Leaf Extracts of Plectranthus amboinicus Lour. Spreng, International Journal of Pharmaceutical Science Invention. 3(2). 19-27. ISSN Online : 2319-6718 ISSN Print : 2319-670X

Brantley, A. U. (2019). Effect of Aqueous Extract of Selected Plants on Liver, Bone and Kidney Markers of 7,12-Dimethylbenz(A)Anthracene (DMBA) Treated Albino-Rats. International Journal of Biochemistry & Physiology, 4(2). doi:10.23880/ijbp-16000150

Boros, L. G., Lerner, M. R., Morgan, D. L., Taylor, S. L., Smith, B. J., Postier, R. G., & Brackett, D. J. (2005). [1,2-13C2]-D-Glucose Profiles of the Serum, Liver, Pancreas, and DMBA-Induced Pancreatic Tumors of Rats. Pancreas, 31(4), 337–343. doi:10.1097/01.mpa.0000186524.53253.fb

Bosha, Joel Aondohulugh, Anaga Aruh Ottah, Asuzu, & Isaac Uzoma. (2015). Bioassay-Guided Isolation and Structural Elucidation of Antidiabetic Principle of Methanol Leaf Extract of Newbouldia laevis (P.Beauv). Journal of Pharmacy and Pharmacology, 3(11). doi:10.17265/2328-2150/2015.11.003

Cheng, J. L., Futakuchi, M., Ogawa, K., Iwata, T., Kasai, M., Tokudome, S., … Shirai, T. (2003). Dose response study of conjugated fatty acid derived from safflower oil on mammary and colon carcinogenesis pretreated with 7,12-dimethylbenz[a]anthracene (DMBA) and 1,2-dimethylhydrazine (DMH) in female Sprague–Dawley rats. Cancer Letters, 196(2), 161–168. doi:10.1016/s0304-3835(03)00280-5

Dakrory, A. I., Fahmy, S. R., Soliman, A. M., Mohamed, A. S., & Amer, S. A. M. (2015). Protective and Curative Effects of the Sea CucumberHolothuria atraExtract against DMBA-Induced Hepatorenal Diseases in Rats. BioMed Research International, 2015, 1–11. doi:10.1155/2015/563652

Hu, Y.q., Wang, J. dan Wu. (2016). Administration of resveratrol enchaces cellcycle arrest followed by apoptosis in DMBA Induced Skin Carcinogenesis in male Wistar rats. European Review for Medical and Pharmacological Sciences. 20(1): 2935-2946.

Kumar, R., Kaur, R., Singh, A. P., & Arora, S. (2014). Diminution of Hepatic Response to 7, 12-dimethylbenz(α)anthracene by Ethyl Acetate Fraction of Acacia catechu Willd. through Modulation of Xenobiotic and Anti-Oxidative Enzymes in Rats. PLoS ONE, 9(2), e90083. doi:10.1371/journal.pone.0090083

Kumar, P., Singh, S., & Kumar, N. (2020). Plectranthus amboinicus: A Review on its Pharmacological and, Pharmacognostical Studies. American Journal of Physiology, Biochemistry and Pharmacology, 10(2), 55. doi:10.5455/ajpbp.20190928091007

Lago, J., Toledo-Arruda, A., Mernak, M., Barrosa, K., Martins, M., Tibério, I., & Prado, C. (2014). Structure-Activity Association of Flavonoids in Lung Diseases. Molecules, 19(3), 3570–3595. doi:10.3390/molecules19033570

Lawrence and A.L. Bacharach. 1964. Evaluation of Drug Activities. Pharmacometrics, Volume 1

Liu, Z., Kundu-Roy, T., Matsuura, I., Wang, G., Lin, Y., Lou, Y.-R., … Liu, F. (2016). Carcinogen 7,12-dimethylbenz[a]anthracene-induced mammary tumorigenesis is accelerated in Smad3 heterozygous mice compared to Smad3 wild type mice. Oncotarget, 7(40). doi:10.18632/oncotarget.11713

Oliveira, K. D. de, Avanzo, G. U., Tedardi, M. V., Rangel, M. M. M., Avanzo, J. L., Fukumasu, H., … Dagli, M. L. Z. (2015). Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts. Brazilian Journal of Veterinary Research and Animal Science, 52(2), 125. doi:10.11606/issn.1678-4456.v52i2p125-133

Patel, R. (2011). Hepatoprotective effects of Plectranthus amboinicus (Lour) Spreng against carbon tetrachloride-induced hepatotoxicity. Journal of Natural Pharmaceuticals, 2(1), 28. doi:10.4103/2229-5119.78495

Rahim, 2010 American Journal of Applied Sciences, 7(9), 1195–1199

Sharmila, R., & Manoharan, S. (2012). Anti-tumor activity of rosmarinic acid in 7,12-dimethylbenz(a)anthracene (DMBA) induced skin carcinogenesis in Swiss albino mice. Indian journal of experimental biology, 50 3, 187-94 .

Silitonga, M., Ilyas, S., Hutahaean, S., & Sipahutar, H. (2014). Levels of Apigenin and Immunostimulatory Activity of Leaf Extracts of Bangunbangun (Plectranthus Amboinicus Lour). International Journal of Biology, 7(1). doi:10.5539/ijb.v7n1p46

Silitonga, M., Gultom, E. S., & Nugrahalia, M. (2020). The Effect of Plectranthus amboinicus Lour Spreng Ethanolic Extract on Relative Organ, Body Weights Changes, and Hematology Profile in Wistar Rats Treated with 7,12Dimethylbenz(a)anthracene. Journal of Physics: Conference Series, 1462, 012001. doi:10.1088/1742-6596/1462/1/012001

Wang, Z., & Zhang, X. (2017). Chemopreventive Activity of Honokiol against 7, 12 - Dimethylbenz[a]anthracene-Induced Mammary Cancer in Female Sprague Dawley Rats. Frontiers in Pharmacology, 8. doi:10.3389/fphar.2017.00320

Zingue, S., Njuh, A. N., Tueche, A. B., Tamsa, J., Tchoupang, E. N., Kakene, S. D., … Njamen, D. (2018). In VitroCytotoxicity andIn VivoAntimammary Tumor Effects of the Hydroethanolic Extract ofAcacia seyal(Mimosaceae) Stem Bark. BioMed Research International, 2018, 1–13. doi:10.1155/2018/2024602




DOI: https://doi.org/10.24114/jbio.v7i3.23288

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JBIO : Jurnal Biosains (The Journal of Biosciences), Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan. ISSN 2443-1230 (print) dan ISSN 2460-6804 (online)

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