.png)
Effect of ethanol leaf extract of Plukenetia volubilis on blood glucose and triglyceride levels of mice induced by alloxan
Abstract
The study aims to determine the phytochemical profile of ethanol extract of kacang saca leaves and its effect on reducing blood glucose and triglyceride levels. Used 18 mice were divided into six groups, namely the normal group, the positive control group (glibenclamide treatment), the negative (alloxan induction), and the treatment group doses used were 0.0262 mg/dL, 0.0525 mg/dL, and 0.105. mg/dL. The treatment of mice was carried out for 10 days with three measurements, namely before treatment, on the 7th day, and on the 10th day of giving the extract. Phytochemical test results showed the presence of flavonoid compounds, alkaloids, steroids, saponins, and tannins. The data obtained were analyzed using the SPSS application. The results of the measurement of blood glucose levels in mice on the 7th day showed normal numbers, the results of data analysis showed no significant difference, namely 0.164. In the measurement of triglycerides, triglyceride levels fell in two groups, namely groups 1 and 3. Data on the 10th day there was an increase in blood glucose and triglycerides. Analysis of the data showed that there was no significant difference in each group on day 7 and day 10. The ethanol extract of cacao beans has the potential to reduce glucose levels used for 7 days but does not affect the triglyceride levels of mice.
Keywords: Alloxan; Glucose; Mice; Triglyceride; Plukenetia volubilis
Full Text:
PDFReferences
Aminah, A., Tomayahu, N., & Abidin, Z. (2017). Penetapan kadar flavonoid total ekstrak etanol kulit buah alpukat (Persea americana Mill.) dengan metode spektrofotometri UV-Vis. Jurnal Fitofarmaka Indonesia, 4(2), 226-230. https://doi.org/10.33096/jffi.v4i2.265
Arifin, A. Y., Ernawati, F., & Prihatini, M. (2019). Hubungan kadar glukosa darah terhadap peningkatan kadar lemak darah pada populasi studi kohor kecamatan Bogor Tengah 2018. Jurnal Biotek Medisiana Indonesia, 8(2), 87-93. https://doi.org/10.22435/jbmi.v8i2.2721
Asri, R. (2020). Pengembangan e-modul pengaruh ekstrak kebiul solid lipid nanoparticle terhadap glukosa darah mencit untuk meningkatkan kemampuan berpikir kritis mahasiswa. Tesis. FKIP Program Pascasarjana S2 Pendidikan IPA, Universitas Bengkulu. Bengkulu.
Huang, Z., Huang, L., Waters, M. J., & Chen, C. (2020). Insulin and growth hormone balance: Implications for obesity. Trends in Endocrinology & Metabolism, 31(9), 642–654. https://doi.org/10.1016/j.tem.2020.04.005
Juwita, R., Saleh, C., & Sitorus, S. (2017). Antihyperuricemia activity test from green leaf of plant red bud (Syzygium myrtifoliumwalp.) to male mice (Mus musculus). Jurnal Atomik, 2(1), 162-168.
Muntafiah, A., Yulianti, D., Cahyaningtyas, A. H., & Damayanti, H. I. (2017). Pengaruh ekstrak jahe merah (Zingiber officinale) dan madu terhadap kadar kolesterol total tikus model diabetes melitus. Scripta Biologica, 4(1), 1-3. https://doi.org/10.20884/1.sb.2017.4.1.329
Nascimento, A. K. L., Melo-Silveira, R. F., Dantas-Santos, N., Fernandes, J. M., Zucolotto, S. M., Rocha, H. A. O., & Scortecci, K. C. (2013). Antioxidant and antiproliferative activities of leaf extracts from Plukenetia volubilis Linneo (Euphorbiaceae). Evidence-Based Complementary and Alternative Medicine, 2013, 1–10. https://doi.org/10.1155/2013/950272
Nordestgaard, B. G. (2017). A test in context: lipid profile, fasting versus nonfasting. Journal of the American College of Cardiology, 70(13), 1637–1646. https://doi.org/10.1016/j.jacc.2017.08.006
Nugrahani, S. S. (2012). Root, stem and leaf extract on meniran to reduce blood glucose level. KEMAS: Jurnal Kesehatan Masyarakat, 8(1), 51-59.
Priyoherianto, A., Ratih Suci, P., Cahya Fatimah, P. R., & Wijayanti, A. N. (2021). Uji aktivitas antidiabetes kombinasi ekstrak etanol daun eceng gondok (Eichhornia crassipes (Mart.) Solms) dan daun sintrong (Crassocephalum crepidioides (Benth.) S. Moore) pada mencit. Jurnal Insan Farmasi Indonesia, 4(1), 44–53. https://doi.org/10.36387/jifi.v4i1.602
Puspitasari, P., & Aliviameita, A. (2018). Relationship between lipid profile and blood glucose levels in diabetes mellitus patients. Medicra (Journal of Medical Laboratory Science/Technology), 1(2), 77-83. https://doi.org/10.21070/medicra.v1i2.1831
Putri, D. K. S. C., Hermanto, B., & Wardani, T. (2014). Effect of infusum salam leaves (Eugenia polyantha) on blood glucose level on the rat (Rattus norwegicus) in alloxan induced. Veterinaria Medika, 7(1), 7-16.
Ramadhani, A., & Probosari, E. (2014). Perbedaan kadar trigliserida sebelum dan setelah pemberian sari bengkuang (Pachyrrhizus erosus) pada wanita, Dissertation, Universtas Diponerogo. Jawa Tengah.
Rasyid, N. Q., Muawanah, M., dan Rahmawati, R. (2018). Gangguan dislipidemia pada pasien diabetes mellitus. In Seminar Nasional Hasil Penelitian & Pengabdian Kepada Masyarakat, p. 149-152.
Simorangkir, M., Sinaga, E., & Silaban, S. (2022). Hepatoprotective and histological pancreas effects of Sarang Banua (Clerodenrum fragrans Vent Willd) leaf extract in alloxan-induced diabetic rats. Rasayan Journal of Chemistry, 15(3), 1846-1854. http://doi.org/10.31788/RJC.2022.1536979
Simorangkir, M., Sinaga, E., Pasaribu, R., & Silaban, S. (2022). Antidiabetic activity of leaf extract of Clerodenrum fragrans Vent Willd in Rattus novergicus induced by alloxan. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 9(1), 119-125. https://doi.org/10.29122/jbbi.v9i1.5264
Siregar, F. A., & Makmur, T. (2020). Metabolisme lipid dalam tubuh. Jurnal Inovasi Kesehatan Masyarakat, 2020, 1(2), 60-66. https://doi.org/10.36656/jikm.v1i2.293
Wahyudi, T., Widyastuti, S. K., & Suarsana, I. N. (2015). Profil lipoprotein plasma tikus dalam kondisi hiperglikemia. Indonesia Medicus Veterinus, 4(2), 116-121.
Wang, S., Zhu, F., & Kakuda, Y. (2018). Sacha inchi (Plukenetia volubilis L.): Nutritional composition, biological activity, and uses. Food Chemistry, 265, 316–328. https://doi.org/10.1016/j.foodchem.2018.05.055
Wardani, E., Sunaryo, H., Sopiani, M. Z., & Fatahillah, M. (2015). Aktivitas antihypertrigliserida dan antihiperglikemik ekstrak daun kelor (Moringa oleifera Lam.) pada tikus hipergliserida diabetes. Media Farmasi, 12(2), 199-212. http://dx.doi.org/10.12928/mf.v12i2.3759
Zhou, J., Kang, X., Luo, Y., Yuan, Y., Wu, Y., Wang, M., & Liu, D. (2019). Glibenclamide-Induced Autophagy Inhibits Its Insulin Secretion-Improving Function in β Cells. International Journal of Endocrinology, 2019, 1–8. https://doi.org/10.1155/2019/1265175
DOI: https://doi.org/10.24114/jpkim.v15i1.42971
Article Metrics
Abstract view : 198 timesPDF - 199 times
Refbacks
- There are currently no refbacks.
Copyright (c) 2023 Dewi Handayani, Zahra Salsabila, Hermansyah Amir, Nurhamidah Nurhamidah, Sura Menda Ginting
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Pendidikan Kimia
Contact: +62 853-1769-2813
Email: jpkim.pps@unimed.ac.id
Jl. Willem Iskandar, Pasar V, Medan Estate, Medan City, North Sumatra Province, Postal Code 20221. Phone/fax: (061) 661 3365 / +62 852-7802-1981.
This work is licensed under a Creative Commons Attribution 4.0 International License.