Characterization and antioxidant assay of yellow frangipani flower (Plumeria alba) extract

Ahmad Fathoni, Tarso Rudiana, Adawiah Adawiah

Abstract


Yellow frangipani flowers (Plumeria alba) are plants that commonly found in the tropics, including Indonesia. Frangipani utilization has not optimum and research about it has not many conducted. This study aims to characterize and test the antioxidant activity of frangipani flower extract. The extraction conducted by maceration method using n-hexane, ethyl acetate, and methanol as solvents. Characterization includes phytochemicals assay (alkaloids, flavonoids, terpenoids, phenolic hydroquinone, tannin-polyphenols, and saponins), total phenolic assay, and total flavonoid assay. Antioxidant test using DPPH radical scavenging assay. The results of the characterization showed that frangipani flower extract positively contained alkaloid, flavonoids, tannins compounds in all solvent phases. Terpenoids are positive in n-hexane and ethyl acetate extract, while saponins are positive in methanol extract. The total phenolic content of n-hexane, ethyl acetate, and methanol extract were 3.6 mgGAE/g; 27.74 mgGAE/g; and 35.23 mgGAE/g. The total flavonoid content of n-hexane, ethyl acetate, and methanol extract were respectively 0.8 mgQE/g; 12.18 mgQE/g; 19.9 mgQE/g. The strongest antioxidant activity is possessed by ethyl extract, followed by methanol extract, and the last are n-hexane extract.

Keywords:

Antioxidant; frangipani flower; phytochemical; total phenolic; total flavonoid

Full Text:

PDF

References


Abdelhady, M. I. S., Motaal, A. A., & Beerhues, L. (2011). Total phenolic content and antioxidant activity of standardized extracts from leaves and cell cultures of three Callistemon species. American Journal of Plant Sciences, 02(06), 847–850, doi: 10.4236/ajps.2011.26100.

AgroMedia (2008). Buku pintar tanaman obat. Jakarta: PT Agromedia Pustaka.

Baba, S. A., & Malik, S. A. (2014). Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science, 9(4), 449–454, doi: 10.1016/j.jtusci.2014.11.001.

Chang, R. (2005). Kimia dasar: Konsep-konsep inti Jilid I. Jakarta: Erlangga.

Febrina, L., Riris, I. D., & Silaban, S. (2017). Activity antibacterial to Escherichia coli and antioxsidant of extract water of leaf binara plant (Artemisia vulgaris L.) after blanching. Jurnal Pendidikan Kimia, 9(2), 311-317, doi: 10.24114/jpkim.v9i2.7621.

Garcia, E. J., Oldoni, T. L. C., Alencar, S. M. D., Reis, A., Loguercio, A. D., & Grande, R. H. M. (2012). Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth. Brazilian Dental Journal, 23(1), 22–27, doi: 10.1590/S0103-64402012000100004.

Guevara, A. P., Amor, E., & Russell, G. (1996). Antimutagens from Plumeria acuminata Ait’, Mutation Research/Environmental Mutagenesis and Related Subjects, 361(2–3), 67–72, doi: 10.1016/S0165-1161(96)90240-X.

Gupta, M., Mazumder, U. K., Gomathi, P., & Selvan, V. T. (2006). Antiinflammatory evaluation of leaves of Plumeria acuminata. BMC Complementary and Alternative Medicine, 6(1), 1–6, doi: 10.1186/1472-6882-6-36.

Harborne, J. B. (1973). Phytochemical methods. Chapman and Hall Ltd. New York: Chapman and Hall, doi: 10.2307/4108146.

John, B. I. J. U., Sulaiman, C. T., George, S., & Reddy, V. R. K. (2014). Total phenolics and flavonoids in selected medicinal plants from Kerala. International Journal of Pharmacy and Pharmaceutical Sciences, 6(1), 406-408.

Latifah (2015). Identifikasi golongan senyawa flavonoid dan uji aktivitas antioksidan pada ekstrak rimpang kencur Kaempferia galanga L. dengan metode DPPH (1,1-Difenil-2-Pikrilhidrazil). Universitas Islam Negeri Maulana Malik Ibrahim Malang. Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Missouri Botanical Garden (2019). Plumeria alba, Plant Finder. Missouri Botanical garden. Available at: http://www.missouribotanicalgarden.org/PlantFinder/PlantFinderDetails.aspx?taxonid=276110&isprofile=0& (Accessed: 24 February 2018).

Nugrahani, R., Andayani, Y., & Hakim, A. (2016). Skrining fitokimia dari ekstrak buah buncis (Phaseolus vulgaris L) dalam sediaan serbuk. Jurnal Penelitian Pendidikan IPA, 2(1), 96–103.

Jaheerunnisa, S., & Chitta, S. K. (2008). Antimicrobial activities of Plumeria acutifolia. Journal of Medicinal Plants Research, 2(4), 077-080.

Rasool, R., Kukal, S. S., & Hira, G. S. (2008). Soil organic carbon and physical properties as affected by long-term application of FYM and inorganic fertilizers in maize–wheat system. Soil and Tillage Research, 101(1-2), 31-36, doi: 10.1016/j.still.2008.05.015.

Robinson, T. (1995). Kandungan organik tumbuhan tinggi. Bandung: ITB.

Situmeang, B., Nuraeni, W., Ibrahim, A. M., & Silaban, S. (2016). Analysis of secondary metabolite compounds from leaves extract kesambi (Schleichera oleosa) and antioxidant activity test. Jurnal Pendidikan Kimia, 8(3), 164-168.

Sukandar, D., Umedi, I. U., Nurbayti, S., Rudiana, T., & Fathoni, A. (2018). Asam protokatekuat dari ekstrak etil asetat biji honje (Etlingera elatior) dan uji aktivitas antioksidannya. Jurnal Kimia Valensi, 4(1), 52-56.

Tristantini, D., Ismawati, A., Pradana, B. T., & Jonathan, J. G. (2016). Pengujian aktivitas antioksidan menggunakan metode DPPH pada daun tanjung (Mimusops elengi L). Prosiding Seminar Nasional Teknik Kimia “Kejuangan”, G1-1-G1-7.

Widjaja, E. A., Rahayuningsih, Y., Rahajoe, J. S., Ubaidillah, R., Maryanto, I., Walujo, E. B., & Semiadi, G. (Eds.). (2014). Kekinian keanekaragaman hayati Indonesia, 2014. LIPI Press, doi: 10.1007/s13398-014-0173-7.2.




DOI: https://doi.org/10.24114/jpkim.v11i1.13034

Article Metrics

Abstract view : 242 times
PDF - 216 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 JURNAL PENDIDIKAN KIMIA

Lisensi Creative Commons
Jurnal Pendidikan Kimia is licensed under a Lisensi Creative Commons Atribusi 4.0 Internasional.

© JURNAL PENDIDIKAN KIMIA. All rights reserved