Phytochemical screening of bunga rosella (Hibiscus sabdariffa L) and antimicrobial activity test

Hermawan Purba, Helen Anjelina Simanjuntak, Risanti Situmorang

Abstract


The purpose of this study was to determine the antimicrobial activity of rosella infusa (Hibiscus sabdariffa L.). Antimicrobial inhibition test using infusa of rosella (Hibiscus sabdariffa L.) against Staphylococcus aureus and Escherichia coli bacteria at a concentration of infusa 100%; 75%; and 50%. The concentration has highest potential antibacterial of infusa for Staphylococcus aureus is 100% witch has diameter of 13.6 mm and for Escherichia coli bacteria is 100%, its show that diameter of zone inhibitory zone is 13.3 mm. The Results of phytochemical screening show that infusa of rosella (Hibiscus sabdariffa L.) contains flavonoids, saponins, and tannins. Based on the data above show that the infusa of rosella flower has high antibacterial potential.

Keywords: Hibiscus sabdariffa L., antimicrobial activity, Staphylococcus aureus, Escherichia coli

Full Text:

PDF

References


Amperawati, S., Puji, H., Yudi, P., & Umar, S. (2019). Efektifitas frekuensi ekstraksi serta pengaruh suhu dan cahaya terhadap antosianin dan daya antioksidan ekstrak kelopak rosella (Hibiscus sabdariffa L.). Jurnal Aplikasi Teknologi Pangan, 8(1), 38–45. doi: 10.17728/jatp.3527.

Ariani, N., Monalisa, M., & Febrianti, D. R. (2019). Uji aktivitas antibakteri ekstrak biji pepaya (Carica Papaya L.) terhadap pertumbuhan Escherichia coli. JCPS: Journal of Current Pharmaceutical Sciences, 2(2), 160-166.

Arifianti, S. R. (2015). Daya antibakteri air rebusan bunga rosella (Hibiscus sabdariffa L.) dan daun teh hitam (Camellia sinensis varian Assamica) terhadap penurunan jumlah koloni bakteri pada sikat gigi. Skripsi. Universitas Jember.

Boucher, H. W., & Corey, G. R. (2008). Epidemiology of methicillin‐resistant Staphylococcus aureus. Clinical Infectious Diseases, 46(S5), S344–S349. doi: 10.1086/533590.

Dalynn. (2014). McFarland Standard. Biological. Catalogue No. TM50-TM60.

Departemen Kesehatan Republik Indonesia. (1995). Materia medika jilid IV. Jakarta: Direktorat Jenderal POM-Depkes RI.

Dhafin. (2017). Analisis cemaran bakteri Coliform escheceria coli pada bubur bayi home industry dikota malang dengan metode TPC dan MPN. Skripsi. Universitas Islam Negeri Malang.

Dinges, M. M., Orwin, P. M., & Schlievert, P. M. (2000). Exotoxins of Staphylococcus aureus. Clinical Microbiology Reviews, 13(1), 16–34. doi: 10.1128/CMR.13.1.16-34.2000.

Faskalia, & Muhamad, A. W. (2014). Skrining fitokimia, uji aktivitas, antioksidan dan uji sitotoksik ekstrak metanol pada akar dan kulit batang soma (Ploiarium alternifolium). Tanjungpura: Fakultas MIPA Universitas Tanjungpura, 3(3).

Febrina, L., Riris, I. D., & Silaban, S. (2017). Activity antibacterial to Escherichia coli and antioxsidant of extract water of leaf binara plant (Artemisia vulgaris L.) after blanching. Jurnal Pendidikan Kimia, 9(2), 311-317. doi: 10.24114/jpkim.v9i2.7621

Franklin L., & Lowy, M. D. (1998). Staphylococcus aureus Infections. The New England Journal of Medicine, 339(8), 520–532. doi: 10.1056/nejm199808203390806

Gurning, K., Siahaan, D., & Iksen, I. (2019). Antibacterial activity test of extract ethanol of jackfruit leaves (Artocarpus heterophyllus Lamk.) of bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis and Salmonella typhi. Journal of Pharmaceutical and Sciences, 2(2), 49-54. doi: 10.36490/journal-jps.com.v2i2.28

Gurning, K. (2020). Antimicrobial activity of ethanol extract of rhizome turmeric (Curcuma longa L.) for growth of Escherichia coli, Staphylococcus aureus and Candida albicans. Asian Journal of Pharmaceutical Research and Development, 8(3), 5-8. doi: 10.22270/ajprd.v8i3.712

Halimah, H., Suci, D. M., & Wijayanti, I. (2019). Studi potensi penggunaan daun mengkudu (Morinda citrifolia L.) sebagai bahan antibakteri Escherichia coli dan Salmonella typhimurium. Jurnal Ilmu Pertanian Indonesia, 24(1), 58-64. doi: 10.18343/jipi.24.1.58.

Jenul, C., & Horswill, A. R. (2018) .Regulation of Staphylococcus aureus Virulence. Microbiology Spectrum, 6. doi: 10.1128/9781683670131.ch41.

Paristiowati, M., Moersilah, M., Stephanie, M. M., Zulmanelis, Z., Idroes, R., & Puspita, R. A. (2019). Rosa sp and Hibiscus sabdariffa L extract in ethanol fraction as acid base indicator: Application of green chemistry in education. J. Phys.: Conf. Ser. 1402 055041. doi: 10.1088/1742-6596/1402/5/055041.

Rahmah, N. F. (2017). Pengaruh pemberian the rosella terhadap penurunan kadar asam urat darah pada penderita asam urat di dusun Ngerambit Desa Kedungbanjar Kecamatan Sugio Kabupaten Lamongan. Skripsi. Universitas Nusantara PGRI Kediri.

Ramadhani, F. (2015). Uji aktivitas antibakteri dari ekstrak etanol 96% kulit batang kayu jawa (Lannea coromandelica) terhadap bakteri Staphylococcus aureus, Escherichia coli, Helicobacter pylori, Pseudomonas aeruginosa. Skripsi. UIN Syarif Hidayatullah Jakarta.

Rasigade, J. P., & Vandenesch, F. (2014). Staphylococcus aureus: a pathogen with still unresolved issues. Infection, Genetics and Evolution, 21, 510-514. doi: 10.1016/j.meegid.2013.08.018.

Schmitt, M., Schuler-Schmid, U., & Schmidt-Lorenz, W. (1990). Temperature limits of growth, TNase and enterotoxin production of Staphylococcus aureus strains isolated from foods. International Journal of Food Microbiology, 11(1), 1–19. doi: 10.1016/0168-1605(90)90036-5.

Siddiq, H. B. H. F. (2019). Sintesis dan uji aktivitas antibakteri senyawa turunan amoksisilin terhadap bakteri Salmonella typhi. Media Pharmaceutica Indonesiana (MPI), 2(2), 90. doi: 10.24123/mpi.v2i2.1319.

Simanjuntak, H.A. (2020). Antibacterial activity of ethanolic extract of kitolod (Hippobroma longiflora) Leaf Against Staphylococcus aureus, and Salmonella typhi. Asian Journal of Pharmaceutical Research and Development. 8(1), 52 -54. doi: 10.22270/ajprd.v8i1.660

Simaremare, E. S. (2014). Skrining fitokimia ekstrak etanol daun gatal (Laportea decumana (Roxb.) Wedd). Pharmacy. Jayapura: Fakultas MIPA Universitas Cendrawasih Jayapura, 11 (1).

Sitorus, P. (2018). Obat Herbal Indonesia (Herbal Medicine). Medan: USU Press

Tekerek, S., Kudret, A., & Alver, Ü. (2011) .Dye-sensitized solar cells fabricated with black raspberry, black carrot and rosella juice. Indian Journal of Physics, 85(10), 1469–1476. doi: 10.1007/s12648-011-0166-8.

Vaghasiya, Y., Dave, R., & Chanda, S. (2011). Phytochemical analysis of some medicinal plants from western region of India. Research Journal of Medicinal Plants, 5(5), 567-576. doi: 10.3923/rjmp.2011.567.576

Wu T, Mengying H, Xixi Z, Ying Z, Tianfu Q, Siyi P, & Xiaoyun X. (2013). A structur activity relationship study of flavonoids as inhibitors of E. coli by membrane interaction effect. Biochimica et Biophysica Acta, 1828(11), 2751-27566. doi: 10.1016/j.bbamem.2013.07.029

Zen, N. A. M., de Queljoe, E., & Singkoh, M. (2015). Uji bioaktivitas ekstrak padina australis dari pesisir pantai molas Sulawesi Utara terhadap bakteri Staphylococcus epidermidis. Jurnal Pesisir dan Laut Tropis, 3(2), 34-40. doi: 10.35800/jplt.3.2.2015.10451




DOI: https://doi.org/10.24114/jpkim.v12i2.19398

Article Metrics

Abstract view : 879 times
PDF - 692 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Hermawan Purba, Helen Anjelina Simanjuntak, Risanti Situmorang

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Pendidikan Kimia
Contact: +62 853-1769-2813
Email: jpkim.pps@unimed.ac.id

Jl. Willem Iskandar, Pasar V, Medan Estate, Medan City, North Sumatra Province, Postal Code 20221. Phone/fax: (061) 661 3365 / +62 852-7802-1981.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.