Synthesis of silver nanoparticles from coffee parasite leaf extract (Scurrulla ferruginea (Roxb. ex Jack) Danser) and potential activity as an antioxidant and anticancer

Destria Roza, Tico Guinessha Samosir, Sri Adelila Sari, Rini Selly

Abstract


This study aim is to know the results of the synthesis of silver nanoparticles from quercetin and Scurrula ferruginea (Roxb. Ex Jack) danser as well as its potential as an antioxidant and anticancer. The solvent used in the maceration extraction method in this research is the polar solvent methanol. Isolation and purification of thick extract compounds from coffee parasite leaves were carried out using liquid vacuum chromatography (CVC) and gravity column chromatography (GCC) methods. Silver (Ag) nanoparticle is made with the stirrer method at a speed of 1500 rpm for 60 minutes at a temperature of 70o C. Silver nanoparticle solution was produced and then characterized using FTIR (Fourier Transform Infra Red) and PSA (Particles Size Analyzer). PSA results analysis using extract coffee parasite size 214 nm particles, whereas using flavonoid isolate quercetin confirmed size 29 nm. FTIR showed that there was a stretching vibration of the OH group at a wave number of 3229.25 cm-1, the C=O function at the wave number 1637.87 cm-1, and the CO functional group at the wave number 1016.31 cm-1. Antioxidant test using the DPPH method was carried out in silver nanoparticle solution using extract Scurrula ferruginea (Roxb. Ex Jack) danser and with quercetin obtained an IC50 value of around 115.4 and 114.9 this result describes as moderate level of antioxidants. Even though the nanoparticle anticancer test was very weak, overall, the synthesis of silver nanoparticles using flavonoid isolates was better than using extracts from coffee parasite leaves.

Keywords


Antioxidant test; Flavonoid isolate; Scurrula ferruginea (Roxb. Ex Jack) danser; Silver (Ag) nanoparticle

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Almudhafar, S. M. A., & Al-Hamdani, M. A. (2022). Antibacterial and Anticancer Effects of Silver Nanoparticles Synthesised using Eragrostis tef and Vitellaria paradoxa Seeds Extract. Basrah Journal of Agricultural Sciences, 35(2), 132–159. https://doi.org/10.37077/25200860.2022.35.2.10

Ameer, O. Z., Salman, I. M., Siddiqui, M. J. A., Yam, M. F., Sriramaneni, R. N., Sadikun, A., Ismail, Z., Shah, A. M., & Asmawi, M. Z. (2010). Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. Brazilian Journal of Medical and Biological Research, 43(2), 186–194. https://doi.org/10.1590/s0100-879x2010005000002

Begum, Q., Kalam, M., Kamal, M., & Mahboob, T. (2020). Biosynthesis, characterization, and antibacterial activity of silver nanoparticles derived from Aloe barbadensis Miller Leaf Extract. Iranian Journal of Biotechnology, 18(2), e2383. https://doi.org/10.30498%2FIJB.2020.145075.2383

Coates, J. (2006). Interpretation of Infrared Spectra. A Practical Approach: Encyclopedia of Analytical Chemistry, John Wiley & Sons, Ltd.

Hong, X., Mat Isa, N., Fakurazi, S., & Safinar Ismail, I. (2019). Phytochemical and anti‐inflammatory properties of Scurrula ferruginea (Jack) Danser parasitising on three different host plants elucidated by NMR‐based metabolomics. Phytochemical Analysis, 31(1), 15–27. Portico. https://doi.org/10.1002/pca.2861

Ijaz, I., Gilani, E., Nazir, A., & Bukhari, A. (2020). Detail review on chemical, physical and green synthesis, classification, characterizations and applications of nanoparticles. Green Chemistry Letters and Reviews, 13(3), 223–245. https://doi.org/10.1080/17518253.2020.1802517

Juwitaningsih, T., Roza, D., Silaban, S., Hermawati, E., & Windayani, N. (2022). Phytochemical screening, antibacterial, antioxidant, and anticancer activity of Coffee parasite acetone extract (Loranthus ferrugineus Roxb). Pharmacia, 69(4), 1041–1046. https://doi.org/10.3897/pharmacia.69.e91427

Kajani, A.A., Zarkesh-Esfahani, S.H., Bordbar, A.K., Khosropour, A.R., Razmjou, A., and Kardi, M. (2016). Anticancer effects of silver nanoparticles encapsulated by Taxus baccata extracts, J Mol Liq, 223. https://doi.org/10.1016/j.molliq.2016.08.064

Kajani, A. A., Zarkesh-Esfahani, S. H., Bordbar, A.-K., Khosropour, A. R., Razmjou, A., & Kardi, M. (2016). Anticancer effects of silver nanoparticles encapsulated by Taxus baccata extracts. Journal of Molecular Liquids, 223, 549–556. https://doi.org/10.1016/j.molliq.2016.08.064

Lestari, G. A. D., Suprihatin, I. E., & Sibarani, J. (2019). Synthesis of silver nanoparticles (NPAg) using Andaliman (Zanthoxylum acanthopodium DC.) fruit water extract and its application in indigosol blue photodegradation. Jurnal Kimia Sains dan Aplikasi, 22(5), 200–205. https://doi.org/10.14710/jksa.22.5.200-205

Marvibaigi, M., Amini, N., Supriyanto, E., Jamil, S., Abdul Majid, F. A., & Khangholi, S. (2014). Total phenolic content, antioxidant and antibacterial properties of Scurrula ferruginea Extracts. Jurnal Teknologi, 70(5). https://doi.org/10.11113/jt.v70.3517

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol, 26(2), 211-219.

Mu, Y., Fu, Y., Li, J., Yu, X., Li, Y., Wang, Y., Wu, X., Zhang, K., Kong, M., Feng, C., & Chen, X. (2019). Multifunctional quercetin conjugated chitosan nano-micelles with P-gp inhibition and permeation enhancement of anticancer drug. Carbohydrate Polymers, 203, 10–18. https://doi.org/10.1016/j.carbpol.2018.09.020

Ozgen, S., Kilinc, O. K., & Selamoğlu, Z. (2016). Antioxidant activity of quercetin: A mechanistic review. Turkish Journal of Agriculture - Food Science and Technology, 4(12), 1134. https://doi.org/10.24925/turjaf.v4i12.1134-1138.1069

Roza, D., Betria Sinaga, N., & Ambarita, C. (2022a). Isolation of secondary metabolite compounds of Coffee Benalu leaves (Loranthus parasiticus (L.) Merr.) and its antibacterial activity test. Jurnal Pendidikan Kimia, 14(2), 111–119. https://doi.org/10.24114/jpkim.v14i2.33706

Roza, D., Selly, R., Hayati, D. Z., & Fadhilah, G. (2022). Identification and antioxidant activity test for fractionated compound from ethanol extract of the singkut rhizome (Molineria latifolia Dryand). RASAYAN Journal of Chemistry, 15(02), 1035–1039. https://doi.org/10.31788/rjc.2022.1526760

Roza, D., Selly, R., & Juwitaningsih, T. (2023). Identification and anti-cancer activity of polar isolates leaf of coffee parasite (Scurulla ferruginea (roxb. ex jack) danser. The 9th Annual International Seminar on Trends in Science and Science Education (AISTSSE), 236–242. https://doi.org/10.2478/9788367405195-035

Xu, R., Wang, D., Zhang, J., & Li, Y. (2006). Shape‐Dependent Catalytic Activity of Silver Nanoparticles for the Oxidation of Styrene. Chemistry – An Asian Journal, 1(6), 888–893. Portico. https://doi.org/10.1002/asia.200600260

Yan, L., Shen, J., Wang, J., Yang, X., Dong, S., & Lu, S. (2020). Nanoparticle-based drug delivery system: A patient-friendly chemotherapy for oncology. Dose-Response, 18(3), 155932582093616. https://doi.org/10.1177/1559325820936161

Yusof, K. N., Alias, S. S., Harun, Z., Basri, H., & Azhar, F. H. (2018). Parkia speciosa as reduction agent in green synthesis silver nanoparticles. Chemistry Select, 3(31), 8881–8885. Portico. https://doi.org/10.1002/slct.201801846




DOI: https://doi.org/10.24114/jpkim.v16i1.54145

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