Antimicrobial and toxicity tests of flavonoid total Dendrophthoe pentandra (L) miq from false ashoke tree (Polyalthia longifolia)

Aliyah Fahmi, Rumondang Bulan Nasution, Hamonangan Nainggolan

Abstract


Abstract:           

Research on antimicrobial and toxicity tests of flavonoid total Dendrophthoe pentandra (L) Miq from false ashoke tree (Polyalthia longifolia) have been done. This study aims to determine the activity of toxicity of total flavonoid of Dendrophthoe pentandra (L) Miq as mistletoe of false ashoke tree (P. longifolia) based on Brine Shrimp Lethality Test (BSLT) method using Artemia salina Leach shrimp larvae obtained Lethal Concentration (LC50) of 30.06 mg / L which means strong toxic activity. Antimicrobial test of flavonoid total has used the diffusion method in order to obtain the inhibit zone diameter on Streptococcus mutans at the total flavonoids concentration of 3%, 6% and 9% were 6; 9 and 17.25 mm, in Escherichia coli is 3.55; 4.25 and 9.15 mm and at Candida albicans is 8.30; 4 and 5.30 mm where S. mutans and E. coli are greater but C. albicans less affect but still have good inhibitory activity so effective to be developed as  antimicrobial agents.

Keywords:

false ashoke leaves, antimicrobial, toxicity, diffusion and BSLT

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References


Davis, W.W., & Stout, T.R. (1971). Disc Plate Method of Microbiological Antibiotic Assay I. Factors Influencing Variability and Error. Applied Microbiology. 22, 659-665.

Detroit Digestive Ferments Company. (1985). Difco manual: dehydrated culture media and reagents for microbiology. Difco laboratories.

Fatmawati, D.A. (2008). Pola Protein dan Kandungan Kurkuminoid Rimpang Temulawak (Curcuma Xanthorrhiza Roxb.). FMIPA. ITB. Bandung.

Harborne, J.B. (1987). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan Edisi Kedua. Bandung: ITB.

Lakshmi, A.P.A.R.N.A., Rao, Y., Bhargavi, C., & Seelam, U. (2011). Antidiabetic and Wound Healing Activity of Various Bark Extracts of Polyalthialongifolia. Asian Journal of Pharmaceutical and Clinical Research. 4, 109-113.

Madigan, M.T., Martinko, J. M., & Parker, J. (2017). Brock biology of microorganisms. (vol. 13) Pearson.

Malairajan, P., Gopalakrishnan, G., Narasimhan, S., & Veni, K.J.K. (2008). Evalution of anti-ulcer activity of Polyalthia longifolia (Sonn.) Thwaites in experimental animals. Indian J Pharmacol. 40, 126–128.

Manjula, S.N., Kenganora, M., Parihar, V.K., Kumar, S., Nayak, P.G., Kumar, N., & Rao, C.M. (2010). Antitumor and antioxidant activity of Polyalthia longifolia stem bark ethanol extract. Pharmaceutical biology. 48, 690-696.

Mclaughlin, J.L., Rogers, L.L., & Anderson, J.E. (1998). The use of biological assays to evaluate botanicals. Drug information journal. 32, 513-524.

Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.J., & McLaughlin, J.L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica. 45, 31-34.

Nuria, M.C., & Faizatun, A. (2009). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas L) Terhadap Bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408. MEDIAGRO. 5, 26-37.

Pitojo, S. (1996). Benalu hortikultura: Pengendalian dan pemanfaatan. Trubus Agriwidya, Ungaran.

Purnomo, B. (2000). Uji Ketoksikan Akut Fraksi Etanol Daun Benalu (Dendropthae Sp) Pada Mencit Jantan Dan Uji Kandungan Kimia. Fakultas Farmasi Universitas Gadjah Mada. Yogyakarta.

Rastogi, R.P., & Mehrotra, B.N. (1995). Compendium of Indian medicinal plants. New Delhi: CDRI.

RI, D.J.P. (2000). Parameter Standarisasi Umum Ekstrak Tumbuhan Obat. Departemen Kesehatan Republik Indonesia, Jakarta. 1, 10-11.

Sari, F.P., & Sari, S.M. (2011). Ekstraksi Zat Aktif Antimikroba dari Tanaman Yodium (Jatropha multifida Linn) sebgai Bahan Baku Alternatif Antibiotik Alami. Semarang: Fakultas Teknik Universitas Diponegoro.




DOI: https://doi.org/10.24114/jpkim.v10i1.9677

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