Crystal guava (Psidium guajava L. “Crystal”) treated with gibberelic acid (GA3) : Evaluation of in vitro antioxidant capacities

Fajar Sumi Lestari, Ricson Pemimpin Hutagaol, Srikandi Srikandi

Abstract


Free radicals can cause many diseases, such as cancer. Antioxidant is a compound that could scavenge free radicals. One of the natural source antioxidants is guava. The application of gibberellic acid (GA3) is known to affect the physicochemical properties and antioxidant content of the fruit. The goals of this research were to investigate the antioxidant activity of skin, flesh and fruit of crystal guava were given additional GA3 treatment with concentration 0; 60; 120; 180; 240; and 300 ppm by determining the value of the Antioxidant Activity Index (AAI) using DPPH; evaluate the total phenolic content (TPC), ferric reducing antioxidant power (FRAP) and physicochemical properties; analyse the correlation between the TPC with AAI DPPH by Pearson’s method. Extraction was performed by the maceration method using acetone 50% mixed with a magnetic stirrer for 1 h. Determination of AAI DPPH, FRAP and the TPC was performed by UV-Visible spectrophotometry. TPC in the flesh and fruit fractions tended to increase and FRAP values in all fractions decreased with GA3 application. The highest TPC and FRAP was given by the G3 treatment on the skin fraction and G0 treatment on the skin fraction. The highest AAI in all fractions was given by the G3 treatment. The TPC was significantly positively correlated with the AAI DPPH of the flesh and fruit fraction extracts (0.250 ≤ r ≤ 0.880), and the skin fraction extracts showed a strong negative correlation between TPC and AAI DPPH (-0.813 < r < -0.973).

Keywords: Antioxidant activity; DPPH; FRAP; Gibberellic acid; Psidium guajava


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Adnyesuari, A. A., Murti, R. H., & Mitrowihardjo, S. (2015). Induksi partenokarpi pada tiga genotipe tomat dengan GA3. Ilmu Pertanian (Agricultural Science), 18(1), 56-62. https://doi.org/10.22146/ipas.6504

Awad, M. A., & Al-Qurashi, A. D. (2012). Gibberellic acid spray and bunch bagging increase bunch weight and improve fruit quality of ‘Barhee’ date palm cultivar under hot arid conditions. Scientia Horticulturae, 138, 96–100. https://doi.org/10.1016/j.scienta.2012.02.015

Bouchoukh, I., Hazmoune, T., Boudelaa, M., Bensouici, C., & Zellagui, A. (2019). Anticholinesterase and antioxidant activities of foliar extract from a tropical species: Psidium guajava L. (Myrtaceae) grown in Algeria. Current Issues in Pharmacy and Medical Sciences, 32(3), 160–167. https://doi.org/10.2478/cipms-2019-0029

Chang, C.-H., Lin, H.-Y., Chang, C.-Y., & Liu, Y.-C. (2006). Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. Journal of Food Engineering, 77(3), 478–485. https://doi.org/10.1016/j.jfoodeng.2005.06.061

Dagar, A., Weksler, A., Friedman, H., & Lurie, S. (2012). Gibberellic acid (GA3) application at the end of pit ripening: Effect on ripening and storage of two harvests of ‘September Snow’ peach. Scientia Horticulturae, 140, 125–130. https://doi.org/10.1016/j.scienta.2012.03.013

Dewage, D. D. D. H., Samarakoon, S. M. G. K., Karunaratne, S. H. S., & Rajapakse, C. S. K. (2022). Psidium guajava L. (Common Guava) peel, pulp and leaves as natural sources of antioxidants, antimicrobials and photoprotective agents for development of sun protection cosmeceuticals. Asian Journal of Chemistry, 35(1), 89–94. https://doi.org/10.14233/ajchem.2023.24058

de Souza Silva, M., Leonel, S., Leonel, M., Souza, J. M. A., Ferreira, R. B., & Martins, R. C. (2022). Plant growth regulators as thinners in guava orchards increase larger fruits with higher nutritional value. Emirates Journal of Food and Agriculture, 34(2), 107-116. https://doi.org/10.9755/ejfa.2022.v34.i2.2815

Fadda, A., Serra, M., Molinu, M. G., Azara, E., Barberis, A., & Sanna, D. (2014). Reaction time and DPPH concentration influence antioxidant activity and kinetic parameters of bioactive molecules and plant extracts in the reaction with the DPPH radical. Journal of Food Composition and Analysis, 35(2), 112–119. https://doi.org/10.1016/j.jfca.2014.06.006

Fidrianny, I., Winarsih, S., & Ruslan, K. (2017). Phytochemical content and antioxidant potential of different organs of Eggplant (Solanum melongena L.) grown in West Java-Indonesia. Asian Journal of Pharmaceutical and Clinical Research, 10(8), 144. https://doi.org/10.22159/ajpcr.2017.v10i8.18584

Fidrianny, I., Suhendy, H., & Insanu, M. (2018). Correlation of phytochemical content with antioxidant potential of various sweet potato (Ipomoea batatas) in West Java, Indonesia. Asian Pacific Journal of Tropical Biomedicine, 8(1), 25. https://doi.org/10.4103/2221-1691.221131

Hartati, R., Nadifan, H. I., & Fidrianny, I. (2020). Crystal guava (Psidium guajava L. “Crystal”): Evaluation of in vitro antioxidant capacities and phytochemical content. The Scientific World Journal, 2020, 1–7. https://doi.org/10.1155/2020/9413727

Hamid Musa, K., Abdullah, A., & Subramaniam, V. (2015). Flavonoid profile and antioxidant activity of pink guava. ScienceAsia, 41(3), 149. https://doi.org/10.2306/scienceasia1513-1874.2015.41.149

Hong, Y., Wang, Z., Barrow, C. J., Dunshea, F. R., & Suleria, H. A. R. (2021). High-throughput screening and characterization of phenolic compounds in stone fruits waste by LC-ESI-QTOF-MS/MS and their potential antioxidant activities. Antioxidants, 10(2), 234. https://doi.org/10.3390/antiox10020234

Irawan, C., Hanafi, H., Sulistiawaty, L., & Rochaeni, H. (2017). Phytochemistry and total phenolic content of methanol extract of Pometia pinata J.R. Forst. & G. Forst. fruit flesh from Papua, Indonesia. Tropical Plant Research, 4(3), 401–404. https://doi.org/10.22271/tpr.2017.v4.i3.053

Jamal-Uddin, A. F. M., Hossan, M. J., Islam, M. S., Ahsan, M. K., & Mehraj, H. (2012). Strawberry growth and yield responses to gibberellic acid concentrations. Journal of Experimental Biosciences, 3(2), 51-56.

Kim, J.-S., & Lee, J.-H. (2020). Correlation between Solid Content and antioxidant activities in Umbelliferae Salad Plants. Preventive Nutrition and Food Science, 25(1), 84–92. https://doi.org/10.3746/pnf.2020.25.1.84

Kumar, R., Bakshi, M., & Singh, D. B. (2012). Influence of plant growth regulators on growth, yield and quality of strawberrry (Fragaria x ananassa Duch.) under UP sub tropics. Asian J. Hortic, 7, 434-436.

Kumar, G. V., Kumar, A., Raghu, K., Patel, G. R., & Manjappa, S. (2013). Determination of vitamin C in some fruits and vegetables in Davanagere city, (Karanataka)-India. International Journal of Pharmacy & Life Sciences, 4(3), 2489-2491.

Kumra, R., Saravanan, R. S., Bakshi, P., Kumar, A., Singh, M., & Kumar, V. (2018). Influence of plant growth regulators on strawberry: A review. International Journal of Chemical Studies, 6(1), 1236-1239.

Lal, N., & Das, R. P. (2017). Effect of Plant Growth Regulators on Yield and Quality of Guava (Psidium guajava L.) cv. Allahabad Safeda. International Journal of Current Microbiology and Applied Sciences, 6(5), 857–863. https://doi.org/10.20546/ijcmas.2017.605.096

Liu, X., Yan, X., Bi, J., Liu, J., Zhou, M., Wu, X., & Chen, Q. (2018). Determination of phenolic compounds and antioxidant activities from peel, flesh, seed of guava (Psidium guajava L.). Electrophoresis, 39(13), 1654–1662. https://doi.org/10.1002/elps.201700479

Maisarah, M. H., Noriham, A., & Zainon, M. N. (2013). Quantification of polyphenolic acids and antioxidant capacity of palm puree from different tenera breeds of Elaeis Guineensis. International Journal of Bioscience, Biochemistry and Bioinformatics, 3(4), 349.

Mu’nisa, A. (2012). Analisis kadar likopen dan uji aktivitas antioksidan pada tomat asal Sulawesi Selatan. Jurnal Bionature, 13(1), 62-66.

Musa, K. H., Abdullah, A., Jusoh, K., & Subramaniam, V. (2010). Antioxidant Activity of Pink-Flesh Guava (Psidium guajava L.): Effect of Extraction Techniques and Solvents. Food Analytical Methods, 4(1), 100–107. https://doi.org/10.1007/s12161-010-9139-3

Peng, Y., Bishop, K. S., & Quek, S. Y. (2019). Extraction optimization, antioxidant capacity and phenolic profiling of extracts from flesh, peel and whole fruit of New Zealand Grown Feijoa cultivars. Antioxidants, 8(5), 141. https://doi.org/10.3390/antiox8050141

Praptiwi, P., Sulistiarini, D., Qodrie, E. N. P., & Sahroni, D. (2021). Antibacterial activity, antioxidant potential, total phenolic and flavonoids of three plant species of Rubiaceae from Banggai Island, Indonesia. Biodiversitas Journal of Biological Diversity, 22(5), 2773-2778. https://doi.org/10.13057/biodiv/d220540

Singh, K., Sharma, M., & Singh, S. (2017). Effect of plant growth regulators on fruit yield and quality of guava (Psidium guajava) cv. Allahabad Safeda. Journal of Pure and Applied Microbiology, 11(2), 1149–1154. https://doi.org/10.22207/jpam.11.2.61

Skenderidis, P., Leontopoulos, S., Petrotos, K., & Giavasis, I. (2020). Optimization of vacuum microwave-assisted extraction of pomegranate fruits peels by the evaluation of extracts’ phenolic content and antioxidant activity. Foods, 9(11), 1655. https://doi.org/10.3390/foods9111655

Tian, H., Xu, Y., Liu, S., Jin, D., Zhang, J., Duan, L., & Tan, W. (2017). Synthesis of gibberellic acid derivatives and their effects on plant growth. Molecules, 22(5), 694. https://doi.org/10.3390/molecules22050694

Wang, L., Clardy, A., Hui, D., Gao, A., & Wu, Y. (2019). Antioxidant and antidiabetic properties of Chinese and Indian bitter melons (Momordica charantia L.). Food Bioscience, 29, 73–80. https://doi.org/10.1016/j.fbio.2019.03.010

Wern, K. H., Haron, H., & Keng, C. B. (2016). Comparison of total phenolic contents (TPC) and antioxidant activities of fresh fruit juices, commercial 100% fruit juices and fruit drinks. Sains Malaysiana, 45(9), 1319-1327.

Yan, L.Y., Teng, L. T., & Jhi, T. J. (2006). Antioxidant properties of guava fruit: comparison with some local fruits. Sunway Academic Journal, 3, 9-20.




DOI: https://doi.org/10.24114/jpkim.v15i2.41780

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